Immunological Exploration of Helicobacter pylori Serotype O_(2) O-Antigen by Using a Synthetic Glycan Library  

作　　者：Ming Zhao Guangzong Tian Chunjun Qin Xiaopeng Zou Peter H. Seeberger Jing Hu Jian Yin 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology & School of Life Sciences and Health Engineering,Jiangnan University,Lihu Avenue 1800,Wuxi,Jiangsu,214122 China [2]Biomolecular Systems Department,Max Planck Institute of Colloids and Interfaces,Am Mühlenberg 1,Potsdam,14476 Germany [3]Wuxi School of Medicine,Jiangnan University,Lihu Avenue 1800,Wuxi,Jiangsu,214122 China

出　　处：《Chinese Journal of Chemistry》2024年第3期243-251,共9页中国化学（英文版）

基　　金：financial support from the National Natural Science Foundation of China(22177041,22277042,22077052,22107037,22207042);the China Postdoctoral Science Foundation(2021M691279);the 111 Project(111-2-06);the Max Planck Society International Partner Group Program,and the China Scholarship Council(CSC)for funding.P.H.S.thanks the Max Plank Society forgenerous financial support.

摘　　要：Helicobacter pylori(H.pylori)infection is a threat to human health.The lipopolysaccharide(LPS)O-antigen holds promise for developing vaccines.It is meaningful to explore the immunological activity of oligosaccharides with different lengths and frameshifts for antigen development.Herein,a glycan library of H.pylori O2 O-antigen containing eight fragments is constructed.After screening with anti-H.pylori O2 LPS sera and patients’sera by glycan microarray,the disaccharide HPO2G-2b and trisaccharide HPO2G-3a show strong antigenicity and then are separately conjugated with carrier protein CRM197.Both glycoconjugates elicit a robust immunoglobulin G(IgG)immune response in rabbits.The anti-HPO2G-3a IgG antibodies possess a much stronger binding affinity with the LPS and bacteria of H.pylori O2 than the anti-HPO2G-2b IgG antibodies.There is no cross-reaction between both sera IgG antibodies with LPS and bacteria of H.pylori O1,O6,and E.coli.The results demonstrate the trisaccharide HPO2G-3a is a promising candidate for H.pylori vaccine development.

关 键 词：GLYCOCONJUGATES Helicobacter pylori Immunology Microarrays O-ANTIGEN OLIGOSACCHARIDES 

分 类 号：R392[医药卫生—免疫学]