双水相萃取茯苓多糖的工艺优化及其抗氧化活性评价  

Optimization of two aqueous phase extraction of Poria polysaccharide and evaluation of its antioxidant activity

作　　者：李琪 李盈盈 罗姣姣 李羿[1] LI Qi;LI Ying-ying;LUO Jiao-jiao;LI Yi(School of Pharmacy,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院药学院,四川成都610500

出　　处：《化学研究与应用》2024年第4期787-794,共8页Chemical Research and Application

基　　金：四川省中医药管理局面上项目(2023MS297)资助;四川省科学技术厅重点研发项目(2020YFN0028)资助。

摘　　要：采用聚乙二醇(PEG)-硫酸铵双水相体系萃取茯苓多糖。通过单因素试验考察和Box-Behnken响应面试验优化萃取工艺条件,得到优化工艺参数为聚乙二醇相对分子质量4000,聚乙二醇质量分数28.46%,硫酸铵质量分数20.60%,此时多糖的萃取率为97.57%。在此条件下,测定茯苓多糖对DPPH·和·OH的清除能力。结果表明茯苓多糖具有体外抗氧化活性,随着茯苓多糖质量浓度的提高,其抗氧化活性增强。A Polyethylene glycol(PEG)-ammonium sulfate aqueous two-phase system was employed for the extraction of Poria polysaccharide.The extraction process conditions were optimized through single-factor experiments and Box-Behnken response surface methodology,and the optimized parameters inclueded a polyethylene glycol molecular weight of 4000,a PEG mass fraction of 28.46%,and an ammonium sulfate mass fraction of 20.60%.Under these conditions,the extraction yield of Poria polysaccharide was 97.57%.Additionally,the determination of the scavenging ability of Poria polysaccharides on DPPH·and·OH.The results showed that Poria polysaccharide had antioxidant activity in vitro,which increased with increasing concentration of Poria polysaccharide.

关 键 词：双水相萃取 茯苓多糖 萃取率 响应面法 抗氧化活性 

分 类 号：O658[理学—分析化学]