新疆桑白皮提取物防龋作用的实验研究  

Experimental study on tanti-caries effect of Xinjiang Mori Cortex extract

作　　者：玛依奴尔·阿塔吾拉 吴龙[1,2] 阿尔曼·阿卜力孜 赵今 Mayinuer Atawula;WU Long;Aerman Abulizi;ZHAO Jin(Department of Cariology and Endodontics,The First Affiliated Hospital of Xinjiang Medical University(The Affiliated Stomatology Hospital of Xinjiang Medical University);Stomatology Disease Institute of Xinjiang Uyghur Autonomous Region,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院(附属口腔医院)牙体牙髓病科 [2]新疆维吾尔自治区口腔医学研究所,乌鲁木齐830054

出　　处：《新疆医科大学学报》2024年第4期455-460,共6页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金(2022D01C751);新疆维吾尔自治区“天山英才”科技领军创新人才项目(2023TSYCLJ0032);新疆医科大学第一附属医院“青年科研起航”专项基金(2022YFY-QKQN-40)。

摘　　要：目的探究新疆桑白皮提取物防龋作用的有效性及安全性。方法建立大鼠龋齿动物模型,将42只SPF-SD大鼠随机分为6组,分别为新疆桑白皮提取物高、中、低剂量组(1.00、0.50、0.25 g/L的新疆桑白皮提取物)、阳性对照组(0.12%的氯己定,CHX组)、阴性对照组(无菌蒸馏水干预的龋病模型组,CA组)、空白对照组(不干预,Control组),各组连续干预大鼠口腔3周。药物有效性评价:采集大鼠唾液并进行变异链球菌的计数,测定变异链球菌水平;采集大鼠颌骨组织,左侧上下颌骨经0.4%紫脲酸铵染色18 h后,体式显微镜下对鼠磨牙的窝沟及光滑面龋进行Keyes评分,评估龋齿的发展程度;将右侧上下颌骨制成牙磨片,经0.1 mmol/L罗丹明B染色24 h后,使用激光共聚焦显微镜观察鼠磨牙牙磨片的脱矿程度,评估龋齿状况。药物安全性评价:记录实验期间各组大鼠的体重增幅,观察新疆桑白皮提取物对大鼠生长发育的影响;计算脏器系数(脏器重量/大鼠体重×100%),评估新疆桑白皮提取物对大鼠主要脏器的影响;取大鼠同一部位颊黏膜,并对大鼠主要脏器、颊黏膜组织进行HE染色,观察组织结构的改变,评估新疆桑白皮提取物对口腔黏膜的刺激性以及脏器细胞的毒性。结果鼠龄50 d,与CA组比较,新疆桑白皮提取物高、中剂量组S.mutans水平降低,差异有统计学意义(P<0.05)。与CA组比较,新疆桑白皮提取物高、中剂量组磨牙光滑面及窝沟处各级龋损Keys评分均降低,新疆桑白皮提取物低剂量组磨牙光滑面、E级、Ds级、Dm级评分均降低,桑白皮高剂量组和CHX组渗透入釉质或牙本质的荧光量降低,新疆桑白皮提取物中剂量及CHX组心脏脏器系数增大,差异有统计学意义(P<0.05)。在实验过程中,各组大鼠健康状况良好,无死亡个体,大鼠体重的生长趋势相似。各组大鼠主要脏器及颊黏膜HE染色切片均匀,组织学结构未见明显改变。结论�Objective To explore the effectiveness and safety of Xinjiang Mori Cortex extract on caries prevention through animal experiments.Methods An animal model of rat caries was established on the basis of previous in vitro studies,42 SPF-SD rats were stochastic divided into 6 groups,respectively Xinjiang Mori Cortex extract high,medium and low dose(1.00 g/L,0.50 g/L,0.25 g/L),positive control group(0.12%chlorhexidine,CHX group),negative control group(sterile distilled water intervention caries model group,CA group),blank control group(no intervention,Control group),by group continuous intervention rats for 3 weeks.(1)Drug effectiveness evaluation:rat saliva was collected,and streptococcus mutans was counted to determine the level of streptococcus mutans.To assess the progression of dental caries,rat jaw tissue was collected and the left upper mandible was stained with 0.4%ammonium violonate for 18 hours,the socket and groove and smooth surface caries of the rat molars were scored under the microscope.The right upper mandible was made into the dental molar piece,stained with 0.1 mmol/L rhodamine B fluorescent dye for 24 hours,and the degree of demineralization of mouse molar teeth was observed by laser confocal microscope.(2)Drug safety evaluation:Recorded the weight increase of therats during the experiment,and observed the effect of Xinjiang Mori Cortex extract on the growth and development of therats.Calculated the coefficient of viscera(Organ weight/rat body weight 100%)to evaluate the effect of Xinjiang Mori Cortex extract on the main organs in rats.HE staining of the main viscera and buccal mucosa tissues wereperformed to observe the changes in tissue structure to evaluate the irritation of Xinjiang Mori Cortex extract on the oral mucosa and organ cytotoxicity.Results After 50 days of mouse age,compared with CA group,the high and medium dose groups of Xinjiang Mori Cortex extract showed a decrease in S.mutans levels,and the difference was statistically significant(P<0.05).Compared with CA group,the high and mediu

关 键 词：新疆桑白皮提取物 防龋 动物模型 有效性 安全性 

分 类 号：R781.4[医药卫生—口腔医学]