灯笼草化学成分的分离和鉴定  

Isolation and identification of chemical constituents from Clinopodium polycephalum

作　　者：苗恬 陶慕珂 周祥敏 刘亚轩 张毅 孟大利 MIAO Tian;TAO Muke;ZHOU Xiangmin;LIU Yaxuan;ZHANG Yi;MENG Dali(School of pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;NMPA Key Laboratory for Quality Monitoring of Narcotic Drugs and Psychotropic Substances,Chongqing Institute for Food and Drug Control,Chongqing 401121,China;School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]国家药品监督管理局麻醉精神药品质量监测重点实验室,重庆市食品药品检验检测研究院,重庆401121 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2024年第4期417-423,492,共8页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81573694);辽宁省教育厅2021年度科学研究经费项目(面上项目,LJKZ0920);重庆市科技局项目(CQYJKJ202001)。

摘　　要：目的 研究唇形科风轮菜属药用植物灯笼草的化学成分。方法 灯笼草干燥全草50.0 kg,粉碎后经体积分数为70%的乙醇溶液回流提取后,综合应用开放柱(硅胶、大孔吸附树脂、凝胶等)色谱和HPLC等方法进行分离及纯化,并鉴定了各分离得到的化合物结构。结果 从灯笼草70%乙醇提取物中分离鉴定了15个化合物,分别为:田蓟苷(tilianin,1)、芹菜素-7-O-β-D-葡萄糖苷(apigenin-7-O-β-D-glucopyranoside,2)、香叶木素-7-O-β-D-葡萄糖苷(diosmetin-7-O-β-D-glucopyranoside,3)、芹菜素-7-O-β-D-葡萄糖醛酸甲酯(apigenin-7-O-β-D-glucuronide methyl ester,4)、木犀草素-7-O-葡萄糖醛酸苷(luteolin-7-O-glucuronide-6″-methyl ester,5)、柚皮素(naringenin,6)、圣草酚(enodicytol,7)、木犀草素(luteolin,8)、芹菜素(apigenin,9)、白桦酯醇(betulin,10)、3-羟基-11-乌苏烯-28,13-内酯(3-hydroxy-11-ursen-28,13-olide,11)、2α,3α-二羟基齐墩果酸(2α,3α-dihydroxyolean-12-en-28-oic acid,12)、常春藤皂苷元(hederagenin,13)、山楂酸(maslinic acid,14)、2α,3α-二羟基熊果酸(2α,3α-dihydroxyursolic acid,15)。结论 化合物1、3、5、7和11为首次从风轮菜属中分离得到。Objective To investigate the chemical constituents of Clinopodium polycephalum.Methods The compounds were isolated from the 70%ethanol/water extract of C.polycephalum using macroporous adsorption resin chromatography,silica gel column chromatography,Sephadex LH-20 chromatography and HPLC techniques,respectively,and identified by their physicochemical properties and spectral technologies.Results Fifteen compounds were successfully isolated and characterized,including tilianin(1),apigenin-7-O-β-D-glucopyranoside(2),diosmetin-7-O-β-D-glucopyranoside(3),apigenin-7-O-β-D-glucuronide methyl ester(4),luteolin 7-O-glucuronide-6″-methyl ester(5),naringenin(6),enodicytol(7),luteolin(8),apigenin(9),betulin(10),3-hydroxy-11-ursen-28,13-olide(11),2α,3α-dihydroxyolean-12-en-28-oic acid(12),hederagenin(13),maslinic acid(14),and 2α,3α-dihydroxyursolic acid(15),respectively.Conclusion Compounds 1,3,5,7,and 11 are isolated from the genus Clinopodium for the first time.

关 键 词：灯笼草 化学成分 黄酮 萜类 

分 类 号：R914[医药卫生—药物化学]