miR-106a通过调控PI3K/PDK1/AKT蛋白通路调节胃癌细胞生物学行为的研究  

miR-106a regulates the biological behavior of gastric cancer cells through the regulation of PI3K/PDK1/AKT pathway

作　　者：李琦[1,2] 黄广智 李亚军 武斌虎 肖茜 阮彩莲 LI Qi;HUANG Guang-zhi;LI Ya-jun;WU Bin-hu;XIAO Xi;RUAN Cai-lian(School of Medicine,Yan'an University,Yan'an,Shaanxi Province 716000,China;Department of General Surgery,Yan'an Hospital of Traditional Chinese Medicine,Yan'an,Shaanxi Province 716000,China)

机构地区：[1]延安大学医学院,陕西延安716000 [2]延安市中医医院普外科,陕西延安716000

出　　处：《解剖学研究》2024年第2期132-138,共7页Anatomy Research

基　　金：陕西省中医药管理局科研课题(SZY-KJCYC-2023-054)。

摘　　要：目的探讨miR-106a对胃癌细胞生物学行为的影响及其作用机制。方法选取AGS人胃癌细胞系,经培养后分为27个样本。所有样本随机分为miR-106a inhibitor、miR-mimic、miR-NC共计3组,分别给予miR-106a抑制剂、miR-106a模拟物及安慰剂干预。观察各组细胞存活率、细胞周期、细胞侵袭、迁移及caspase活性、Bax、Bcl-2、Casepase-3蛋白相对表达量、p85β、p-PDK1、p-AKT蛋白相对表达量。结果与miR-NC组比较,miR-106a inhibitor组AGS细胞活性降低[(分别为15.01±0.97)、(69.82±2.31)%](P<0.01);miR-106a mimics组AGS细胞G0/G1期细胞比例降低(P<0.05)(分别为17.33±1.04、58.24±0.82),G2/M和S期细胞比例升高(分别为50.11±1.12、35.64±1.07和31.56±0.92、9.24±0.25);miR-106a inhibitor组AGS细胞G0/G1期细胞比例升高(分别为78.43±1.12、58.24±0.82)(P<0.05),G2/M和S期细胞比例降低(分别为33.65±0.99、35.64±1.07和19.78±0.84、9.24±0.25)(P<0.01)。与miR-NC相比,miR-106a mimics组AGS细胞的迁移、侵袭能力增强,miR-106a inhibitor组AGS细胞的迁移、侵袭能力减弱(P<0.01);与miR-NC相比,miR-106a mimics组AGS细胞caspase-3、caspase-8、caspase-9活性降低,miR-106a inhibitor组AGS细胞caspase-3、caspase-8、caspase-9活性升高(P<0.05);miR-NC相比,miR-106a mimics组AGS细胞Bax(分别为0.69±0.07、1.48±0.15)、Casepase-3蛋白(分别为0.37±0.04、0.91±0.09)相对表达量降低,Bcl-2蛋白相对表达量升高(分别为1.53±0.12、0.94±0.09),miR-106a inhibitor组AGS细胞Bax(分别为2.07±0.21、1.48±0.15)、Casepase-3蛋白(分别为1.23±0.12、0.91±0.09)相对表达量升高,Bcl-2蛋白相对表达量降低(P<0.05)(分别为0.65±0.07、0.94±0.09);与miR-NC相比,miR-106a mimics组AGS细胞p85β(分别为1.24±0.12、0.94±0.09)、p-PDK1(分别为2.13±0.23、1.01±0.10)、p-AKT蛋白(分别为1.14±0.11、0.72±0.06)相对表达量升高,miR-106a inhibitor组AGS细胞p85β(分别为0.69±0.07、0.94±0.09)、p-PDK1(分别为0.75±0.07、1.01±0.10)、p-AKTObjective To investigate the effects of miR-106a on the biological behavior of gastric cancer cells and its mechanism.Methods Human gastric cancer cell lines of AGS were selected and cultured into 27 sam-ples.All the samples were randomly divided into three groups:miR-106a inhibitor,miR-mimic and miR-NC,which were treated with miR-106a inhibitor,miR-106a mimic and placebo respectively.Cell survival rate,cell cycle,cell invasion,migration,caspase activity,relative expression levels of Bax,Bcl-2,Casepase-3 protein,and relative ex-pression levels of p85β,p-PDK1 and p-AKT protein were observed in each group.Results Compared with miR-NC,AGS cell activity of miR-106a inhibitor group was decreased(15.01±0.97 vs 69.82±2.31)(P<0.01).The pro-portion of G0/G1 phase cells in AGS cells of miR-106a mimics group decreased(P<0.05)(17.33±1.04 vs 58.24±0.82).The proportion of G2/M and S phase cells increased(50.11±1.12 vs 35.64±1.07)(31.56±0.92 vs 9.24±0.25).The proportion of AGS cells in G0/G1 phase was increased(78.43±1.12 vs 58.24±0.82)(P<0.05)after miR-106a in-hibitor treatment.The proportion of G2/M and S phase cells decreased(33.65±0.99 vs 35.64±1.07)(19.78±0.84 vs 9.24±0.25)(P<0.01);Compared with miR-NC,the migration and invasion ability of AGS cells in miR-106a mimics group was enhanced.The migration and invasion ability of AGS cells were decreased vs miR-106a inhibitor group(P<0.01).Compared with miR-NC,the activities of caspase-3,caspase-8 and caspase-9 of AGS cells in miR-106a mimics group were decreased.The activities of caspase-3,caspase-8 and caspase-9 of AGS cells in miR-106a inhibi-tor group were increased(P<0.05).Compared with miR-NC,the relative expression levels of Bax and Casepase-3 in AGS cells of miR-106a mimics group were decreased(0.69±0.07 vs 1.48±0.15)(0.37±0.04 vs 0.91±0.09).The rela-tive expression of Bcl-2 protein was increased(1.53±0.12 vs 0.94±0.09).The relative expression levels of Bax and Casepase-3 in AGS cells of miR-106a inhibitor group were increased(2.07±0.21 vs 1.48±0.1

关 键 词：AGS人胃癌细胞系 miR-106a 磷脂酰肌醇激酶 磷酸肌醇依赖性蛋白激酶-1 蛋白激酶B 癌细胞生物学行为 

分 类 号：R735.2[医药卫生—肿瘤]