E3泛素连接酶三结构域蛋白59对非小细胞肺癌细胞增殖和侵袭的影响  

Effects of E3 ubiquitin ligase TRIM59 on proliferation and invasion of non-small cell lung cancer cells

作　　者：王静怡[1] 马晓林[2] WANG Jing-yi;MA Xiao-lin(Department of Radiotherapy and Thoracic Surgery,Taiyuan,Shanxi Province 030013,China;Department of Tho-racic Surgery,Taiyuan,Shanxi Province 030013,China)

机构地区：[1]山西省肿瘤医院放胸二科,山西太原030013 [2]山西省肿瘤医院胸外科,山西太原030013

出　　处：《解剖学研究》2024年第2期139-144,共6页Anatomy Research

摘　　要：目的探讨三结构域蛋白59(TRIM59)对非小细胞肺癌细胞增殖、迁移和侵袭的影响及作用机制。方法将非小细胞肺癌细胞系A549分为siControl组、siTRIM59组、Flag-Vector组和Flag-TRIM59组,用CCK-8实验检测细胞增殖活力;用TUNEL染色实验检测细胞凋亡率;用划痕实验和Transwell实验检测细胞迁移和侵袭能力;用蛋白质免疫印记实验检测AKT表达水平和磷酸化水平。结果与siControl组相比,siTRIM59组细胞增殖活力下降(P<0.05),凋亡比例升高(P<0.05),细胞迁移率降低(P<0.05),侵袭到Transwell下层小室的细胞数减少(P<0.05),AKT磷酸化水平下降。与Flag-Vector组相比,Flag-TRIM59组细胞增殖活力增强(P<0.05),凋亡比例降低(P<0.05),细胞迁移率升高(P<0.05),侵袭到Transwell下层小室的细胞数增加(P<0.05),AKT磷酸化水平上升。结论TRIM59促进非小细胞肺癌细胞的增殖、迁移和侵袭,以及AKT的磷酸化。Objective To explore the effect and mechanism of TRIM59 on proliferation,migration and invasion of non-small cell lung cancer cells.Methods Non-small cell lung cancer cell line A549 was divided into siControl group,siTRIM59 group,Flag-Vector group and Flag-TRIM59 group.The cell proliferation activity was detected by CCK-8 assay.The apoptosis rate was detected by TUNEL staining assay.The ability of cell migration and invasion was detected by scratch test and Transwell test.AKT expression and phosphorylation levels were detected by immunoimprinting assay.Results Compared with siControl group,cell proliferation activity in siTRIM59 group was decreased(P<0.05),apoptosis ratio was increased(P<0.05),cell mobility was decreased(P<0.05),the number of cells invading the lower chamber of Transwell was decreased(P<0.05),and AKT phosphorylation level was decreased.Compared with Flag-Vector group,cell proliferation activity was enhanced in Flag-TRIM59 group(P<0.05),apoptosis ratio was decreased(P<0.05),cell mobility was increased(P<0.05),the number of cells invading the lower compartment of Transwell was increased(P<0.05),and AKT phosphorylation level was increased.Conclusion TRIM59 promotes proliferation,migration,and invasion of non-small cell lung cancer cells,as well as AKT phosphorylation.

关 键 词：非小细胞肺癌 三结构域蛋白59 增殖 侵袭 蛋白激酶B A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]