实时荧光PCR检测鼠源性成分的定性方法验证  

Validation of Qualitative Methods for Real-time Fluorescence PCR Detection of Mouse Derived Components

作　　者：张从党 王均华 王亚平 张维益 包静云 ZHANG Congdang;WANG Junhua;WANG Yaping;ZHANG Weiyi;BAO Jingyun(Testing Center of Jiangyin Food Safety,Wuxi 214400,China)

机构地区：[1]江阴市食品安全检测中心,江苏无锡214400

出　　处：《现代食品》2024年第4期211-214,共4页Modern Food

摘　　要：本文探讨了GB/T 38164—2019《常见畜禽动物源性成分检测方法实时荧光PCR法》在肉类食品源性成分检测中鼠源性成分检测的可行性和适用性,旨在为动物源性成分的方法验证工作提供参考。结果表明,GB/T 38164—2019的实验方法对大鼠具有较好的特异性,能够准确检测肉类食品中的大鼠鼠源性成分。然而,该标准方法在检测肉类食品中的鼠源性成分时,不具有适用性,需要进一步优化。This article explores the feasibility and applicability of GB/T 38164—2019"Real time Fluorescence PCR Method for Detection of Common Livestock and Poultry Animal Derived Ingredients"in the detection of mouse derived ingredients in meat food,aiming to provide reference for the method validation of animal derived ingredients.The results indicate that the experimental method of GB/T 38164—2019 has good specificity for rats and can accurately detect rat derived components in meat products.However,this standard method is not applicable for detecting mouse derived components in meat products and requires further optimization.

关 键 词：GB/T 38164—2019 鼠源性成分 实时荧光PCR 

分 类 号：TS207.3[轻工技术与工程—食品科学]