痛风石组织中巨噬细胞BCAS3和RFX3蛋白表达及意义  

Expression and significance of BCAS3 and RFX3 protein inmacrophages of gout stone tissue

作　　者：董淑慧[1] 徐瑾[1] DONG Shuhui;XU Jin(Department of Pathology,Tianjin Municipal Tianjin Hospital,Tianjin 300211,China)

机构地区：[1]天津市天津医院病理科,天津300211

出　　处：《重庆医学》2024年第8期1157-1161,共5页Chongqing medicine

基　　金：天津市中西医结合骨科研究所科研基金项目(2019TJGYSKY07)。

摘　　要：目的分析痛风石组织中巨噬细胞人类乳腺癌扩增序列3(BCAS3)和调节因子X3(RFX3)蛋白表达及意义。方法收集2019年4月至2022年12月该院痛风石切除标本30例作为试验组,同期切除的关节置换术后滑膜增生伴有异物巨细胞反应的标本25例、脂肪坏死伴有巨噬细胞增生的标本5例作为对照组,对照组血尿酸均为正常水平。应用免疫组织化学法对所有标本行CD68、CD163、BCAS3和RFX3抗体染色,CD68和CD163用于组织中巨噬细胞的定位,检测巨噬细胞中BCAS3和RFX3蛋白表达,分析两种抗体蛋白表达情况与年龄、性别、血尿酸的相关性,受试者工作特征(ROC)曲线评估二者对于痛风的诊断价值。结果试验组BCAS3、RFX3蛋白表达明显高于对照组(P<0.05),对照组不同性别BCAS3、RFX3蛋白表达比较差异无统计学意义(P>0.05)。试验组BCAS3蛋白表达与RFX3蛋白表达呈正相关(P<0.05)。试验组血尿酸与BCAS3、RFX3蛋白表达均呈正相关(P<0.05),对照组血尿酸与BCAS3、RFX3蛋白表达均不相关(P>0.05)。两组年龄与BCAS3、RFX3蛋白表达不相关(P>0.05)。RFX3的曲线下面积(AUC)、灵敏度、特异度分别为0.935、96.55%、86.67%,均高于BCAS3的0.742、70.00%和80.00%,RFX3对于痛风有更高的诊断价值。结论巨噬细胞高表达BCAS3和RFX3可能参与痛风的发病,二者可能存在某种协同促进作用,RFX3对于痛风的诊断价值高于BCAS3。Objective To analyze the expression and significance of BCAS3 and RFX3 proteins in macrophages from gout stone tissues.Methods Thirty gout stone excision specimens in this hospital from April 2019 to December 2022 were collected as the experimental group,and during the same period 25 specimens of synovial hyperplasia accompanied by foreign body giant cell reaction after joint replacement and 5 specimens of fat necrosis accompanied by macrophage hyperplasia served as the control group.The blood uric acid level of the control group was normal.Immunohistochemical staining was applied to conduct CD68,CD163,BCAS3 and RFX3 antibodies staining in all specimens.CD68 and CD163 were used to locate macrophages in tissues.The expression levels of BCAS3 and RFX3 in macrophages were detected.The expressions of the two antibodies and their correlation with age,sex and blood uric acid level were analyzed.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of the both on gout.Results The expression levels of BCAS3 and RFX3 in the experimental group were significantly higher than those in the control group(P<0.05).The BCAS3 and RFX3 protein expression levels in the control group had no statistical difference between the different sexes(P>0.05).The expression level of BCAS3 protein in the experimental group was positively correlated with the RFX3 protein expression(P<0.05);the uric acid level in the experimental group was positively correlated with the expression level of BCAS3 and RFX3 protein(P<0.05).The blood uric acid level in the control group had no correlation with BCAS3 and RFX3 protein(P>0.05).The age in the two groups had no correlation with the BCAS3 and RFX3 protein(P>0.05).The area under the curve(AUC),sensitivity and specificity of RFX3 were 0.935,96.55%and 86.67%respectively,which were higher than 0.742,70.00%and 80.00%of BCAS3.RFX3 had the higher diagnostic value for gout.Conclusion High expression of BCAS3 and RFX3 proteins in macrophages may participate in the pathogenesis of

关 键 词：痛风 巨噬细胞 人类乳腺癌扩增序列3 调节因子X3 

分 类 号：R365[医药卫生—病理学]