脂联素基因多态性与直肠腺瘤切除预后相关性分析  

Analysis of the correlation between adiponectin gene polymorphismand prognosis of rectal adenoma resection

作　　者：张慧英[1] 刘冰[1] 刘国华 王琳[1] 杨邯平 ZHANG Huiying;LIU Bing;LIU Guohua;WANG Lin;YANG Hanping(Department of General Surgery,Handan First Hospital,Handan Hebei 056000,China)

机构地区：[1]河北省邯郸市第一医院普外科,056000

出　　处：《蚌埠医学院学报》2024年第3期340-343,共4页Journal of Bengbu Medical College

基　　金：河北省医学科学研究重点课题计划(20160338)。

摘　　要：目的:探讨脂联素(APN)基因多态性与直肠腺瘤(RA)切除预后的相关性。方法:选取接受切除手术治疗的RA病人97例,根据预后情况,将复发RA病人52例作为观察组,无复发病人45例作为对照组。通过聚合酶链式反应-限制性片段长度多态性(PCR-RELP)法检测受试者血清中APN基因rs2241766位点多态性基因分布频率,并分析APN单核苷酸多态性各基因型与RA切除预后的相关性。结果:APN基因rs2241766位点基因型有TT野生型、TG杂合子型、GG纯合子型,对照组与观察组APN基因rs2241766位点基因型符合Hardy-Weinberg平衡定律,2组APN基因rs2241766位点基因型及等位基因频率差异均有统计学意义(P<0.01)。突变杂合子TG型、突变纯合子GG型病人的腺瘤数、腺瘤最大径及平均直径均大于野生TT型(P<0.05),纯合子GG型病人腺瘤数、腺瘤最大径及平均直径均大于杂合子TG型(P<0.05)。二元logistic回归分析显示,APN基因rs2241766位点TG型、GG型突变均为RA切除后复发的危险因素(P<0.01)。结论:APN基因rs2241766位点多态性与RA切除预后具有相关性。Objective:To investigate the correlation between adiponectin(APN)gene polymorphism and prognosis of rectal adenoma(RA)resection.Methods:A total of 97 RA patients who underwent resection were selected.According to the prognosis,52 patients with recurrent RA were taken as the observation group and 45 patients without recurrence were taken as the control group.The distribution frequency of polymorphic genes at rs2241766 locus of APN gene was detected by polymerase chain reaction restriction fragment length polymorphism(PCR-RELP),and the correlation between APN single nucleotide polymorphic genotypes and prognosis of RA resection was analyzed.Results:There were TT wild type,TG heterozygote and GG homozygote at rs2241766 locus of APN gene.The genotypes of rs2241766 locus of APN in the control group and the observation group were in accordance with Hardy-Weinberg equilibrium law.There were significant differences in the genotype and allele frequency of rs2241766 locus of APN gene between the two groups(P<0.01).Compared with the wild TT type,the number of adenomas,the maximum and average diameter of adenomas in the mutant heterozygous TG type and the mutant homozygous GG type were significantly increased(P<0.05).Compared with the heterozygous TG type,the number of adenomas,the maximum and average diameter of adenomas in homozygous GG type were significantly increased(P<0.05).Binary logistic regression analysis showed that TG and GG mutations at rs2241766 locus of APN gene were the risk factors for recurrence after RA resection(P<0.01).Conclusions:The rs2241766 polymorphism of APN gene is associated with the prognosis of RA resection.

关 键 词：直肠腺瘤 脂联素 rs2241766位点 基因多态性 预后 

分 类 号：R735.37[医药卫生—肿瘤]