SNORA76在膀胱癌中的表达水平及潜在分子机制  

Expression levels and potential molecular mechanisms of SNORA76 in bladder cancer

作　　者：韦柳萌 黄志广 李生华[2] 陈罡[1] 梁芳芳 龙禹[4] 何融泉 WEI Liu-meng;HUANG Zhi-guang;LI Sheng-hua;CHEN Gang;LIANG Fang-fang;LONG Yu;HE Rong-quan(Department of Pathology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,CHINA;Department of Urology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,CHINA;Department of Medical Oncology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,CHINA;Department of Obstetrics,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,CHINA)

机构地区：[1]广西医科大学第一附属医院病理科,广西南宁530021 [2]广西医科大学第一附属医院泌尿外科,广西南宁530021 [3]广西医科大学第一附属医院肿瘤内科,广西南宁530021 [4]广西医科大学第一附属医院产科,广西南宁530021

出　　处：《海南医学》2024年第8期1065-1070,共6页Hainan Medical Journal

摘　　要：目的探讨膀胱癌组织中SNORA76的表达水平及其潜在分子机制。方法从基因表达数据库(GEO)、癌症基因组图谱(TCGA)公共数据集以及ArrayExpress网站中,获得6个SNORA76在膀胱癌(BLCA)中的表达数据,进行差异基因表达分析和相关性分析,得到的高表达基因与正相关基因、低表达基因与负相关基因分别取交集后得到正共表达基因与负共表达基因,将正共表达基因与负共表达基因分别进行KEGG、GO分析,并进行蛋白-蛋白互作(PPI)网络构建。结果纳入的6个数据集中BLCA组共817例,对照组98例。与对照组相比,SNORA76在BLCA组中高表达(标准化均数差[SMD]=0.48,95%置信区间[CI]=0.26~0.70,P<0.05;曲线下面积[AUC]=0.76,95%CI=0.72~0.80,P<0.05)。SNORA76的正相关基因主要富集在细胞周期通路中,通过蛋白互作网络,筛选出Degree值前十的核心基因TP53、HSP90AA1、HSPA4、FBL、HSPA8、HDAC1、NHP2、RUVBL1、EFTUD2、RPSA。结论SNORA76在膀胱癌中高表达,可能通过影响p53、PI3K-Akt等信号通路参与BLCA的发生发展。Objective To investigate the expression level of SNORA76 in bladder cancer tissues and its poten-tial molecular mechanism.Methods The expression data of six SNORA76 in BLCA were obtained from the Gene Ex-pression Omnibus(GEO),the Cancer Genome Atlas(TCGA)and Array Express website,and the differential gene ex-pression and correlation analysis were performed.The positive co-expression genes and negative co-expression genes were obtained by intersection of the high expression genes and positive correlation genes,and the low expression genes and negative co-expression genes.The positive and negative co-expressed genes were then processed with Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)biological signal pathway enrichment analysis,and the protein-protein interaction(PPI)network was constructed.Results There were 817 patients in the BL-CA group and 98 patients in the control group.Compared with the control group,SNORA76 was highly expressed in the BLCA group[SMD=0.48,95%CI=0.26-0.70,P<0.05;area under the curve(AUC)=0.76,95%CI=0.72-0.80,P<0.05].SNORA76 positively correlated genes were mainly enriched in the cell cycle pathway.Through the protein interaction network,the top ten core genes with degree value were screened out:TP53,HSP90AA1,HSPA4,FBL,HSPA8,HDAC1,NHP2,RUVBL1,EFTUD2,and RPSA.Conclusion SNORA76 is highly expressed in bladder cancer and may be involved in the occurrence and development of BLCA by affecting the p53 and PI3K-Akt signaling pathways.

关 键 词：SNORA76 膀胱癌 富集分析 分子机制 共表达基因 

分 类 号：R737.14[医药卫生—肿瘤]