TLRs介导相关炎症因子表达参与人乳头瘤病毒感染致宫颈癌的作用  

Effect of the expression of TLRs related inflammatory factors on cervical cancer induced by human papillomavirus infection

作　　者：赵卉 张玮璨 张莎莎 王瑞敏[1] 申佳伟 ZHAO Hui;ZHANG Wei-can;ZHANG Sha-sha;WANG Rui-min;SHEN Jia-wei(Xingtai People's Hospital,Xingtai,Hebei 05400l,China)

机构地区：[1]邢台市人民医院妇四科,河北邢台054001

出　　处：《中华医院感染学杂志》2024年第8期1217-1221,共5页Chinese Journal of Nosocomiology

基　　金：河北省医学科学研究重点课题计划(20181652)。

摘　　要：目的探讨Toll样受体(TLRs)介导相关炎症因子表达参与人乳头瘤病毒(hr-HPV)感染致宫颈癌的作用。方法选择2023年1月-2023年12月邢台市人民医院170例hr-HPV感染患者,根据宫颈病变程度分为宫颈炎组50例,低度鳞状上皮内病变(LSIL)组50例,高度鳞状上皮内病变(HSIL)组50例,宫颈癌组20例,检测宫颈组织中TLRs信号通路相关基因、白细胞介素(IL)-6、IL-10、肿瘤坏死因子(TNF-α)、高迁移率族蛋白1(HMGB1)表达。结果与宫颈炎组比较,LSIL组、HSIL组、宫颈癌组TLR4、TLR7、TLR9、核转录因子-κB(NF-κB)基因表达、IL-6、IL-10、TNF-α、HMGB1水平均有不同程度升高,TLR3、IFN-β基因表达水平有不同程度降低(P<0.05);与LSIL组比较,HSIL组、宫颈癌组TLR4、TLR7、TLR9、NF-κB基因表达水平均有不同程度升高,TLR3、IFN-β基因表达水平有不同程度降低(P<0.05);与HSIL组比较,宫颈癌组TLR4、TLR7、TLR9、NF-κB基因表达、IL-6、IL-10、TNF-α、HMGB1、CD_(8)^(+)水平升高,TLR3、IFN-β基因表达、CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水平降低(P<0.05);与LSIL组比较,HSIL组、宫颈癌组IL-6、IL-10、TNF-α、HMGB1、CD_(8)^(+)水平均有不同程度升高,CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)均有不同程度降低(P<0.05);与宫颈炎组比较,LSIL组、HSIL组、宫颈癌组CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)均有不同程度降低,CD_(8)^(+)有不同程度升高(P<0.05)。结论hr-HPV可能参与了宫颈TLRs信号通路激活,介导相关炎症因子分泌,抑制CD_(3)^(+)、CD_(4)^(+)分化,并通过细胞介导的免疫反应抑制,促进宫颈疾病的进展。OBJECTIVE To investigate the role of the expression of Toll-like receptors(TLRs)mediating related in-flammatory factors involved in cervical cancer induced by human papillomavirus(hr-HPV)infection.METHODS A total of 170 patients with hr-HPV infection in Xingtai People's Hospital from Jan 2023 to Dec 2023 were en-rolled in the study.According to the varying degrees of cervical lesions,they were divided into the cervicitis group(50 cases),low-grade squamous intraepithelial lesions(LSIL)group(50 cases),and high-grade squamous intra-epithelial lesions(HSIL)group(50 cases).The expression of TLRs signaling pathway related genes,including in-terleukin(IL)-6,IL-10,tumor necrosis factor(TNF-α)and high mobility group protein 1(HMGB1)were detec-ted in cervical tissues of 20 patients with cervical cancer.RESULTS Compared with the cervicitis group,the ex-pression levels of TLR4,TLR7,TLR9,nuclear transcription factor(NF-κB),IL-6,IL-10,TNF-αand HMGB1 in the LSIL group,HSIL group and cervical cancer group increased to varying degrees,while the expression levels of TLR3 and IFN-βdecreased(P<0.05).Compared with the LSIL group,the expression levels of TLR4,TLR7,TLR9 and NF-κB genes in the HSIL group and cervical cancer group increased to different extent,while the ex-pression levels of TLR3 and IFN-βgenes decreased(P<0.05).Compared with the HSIL group,the gene expres-sion of TLR4,TLR7,TLR9,NF-κB,the levels of IL-6,IL-10,TNF-α,HMGB1 and CD_(8)^(+)in the cervical cancer group increased,while the gene expression of TLR3 and IFN-β,the levels of CD_(3)^(+),CD_(4)^(+)and CD_(4)^(+)/CD_(8)^(+)de-creased(P<0.05).Compared with the LSIL group,the levels of IL-6,IL-10,TNF-α,HMGB1 and CD_(8)^(+)in the HSIL group and cervical cancer group were increased to varying degrees,while the levels of CD_(3)^(+),CD_(4)^(+)and CD_(4)^(+)/CD_(8)^(+)were decreased(P<0.05).Compared with the cervicitis group,CD_(3)^(+),CD_(4)^(+)and CD_(4)^(+)/CD_(8)^(+)in the LSIL group,HSIL group and cervical cancer group decreased to varying degrees,while CD_(8)^

关 键 词：TOLL样受体 炎症因子 人乳头瘤病毒 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]