竹盐对便秘小鼠的影响及其机制研究  

Effects of Bamboo Salt on Constipated Mice and Its Mechanism

作　　者：肖敏 刘达 游秋云 但汉雄 尤朋涛 XIAO Min;LIU Da;YOU Qiuyun;DAN Hanxiong;YOU Pengtao(Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine,College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065;Wuhan Green Time Creative Technology Co.,Ltd.,Wuhan 430012)

机构地区：[1]湖北中医药大学药学院,中药资源与中药化学省级重点实验室,武汉430065 [2]武汉绿时代创新科技有限公司,武汉430012

出　　处：《食品工业》2024年第3期124-129,共6页The Food Industry

摘　　要：研究竹盐对盐酸洛哌丁胺诱导的慢传输型便秘(STC)小鼠模型的缓解作用和机制。对小鼠连续7 d灌胃盐酸洛哌丁胺(10 mg/kg)造便秘模型,第8天开始,灌胃盐酸洛哌丁胺1 h后,再灌胃低中高剂量(0.5,1.0和1.5 g/kg)竹盐,2.5mg/kg枸橼酸莫沙必利和生理盐水。第14天灌胃结束后统计6 h排便粒数,测量小肠计算肠推进率。采用苏木精-伊红染色法观察结肠病理学变化。采用试剂盒测定血清中胃泌素(Gas)、P物质(SP)和一氧化氮(NO)含量。采用蛋白质印迹法测定结肠中c-kit原癌基因(c-kit)、血管活性肠肽(VIP)、蛋白激酶A (PKA)、水通道蛋白3 (AQP3)蛋白的表达,采用实时荧光定量法测定结肠组织中干细胞因子(SCF)和环磷酸腺苷(cAMP)的mRNA的相对表达。结果显示:与模型组相比,竹盐中高剂量组和枸橼酸莫沙必利组中STC小鼠结肠组织肌层逐渐变厚,杯状细胞增多,缓解了便秘导致的黏膜损伤;与模型组相比,中高剂量竹盐和枸橼酸干预后明显增加了STC小鼠肠推进率和血清中Gas和SP含量,减少了NO含量,促进了结肠组织中c-kit、VIP、PKA、AQP3蛋白表达水平和SCF、cAMP的mRNA的相对表达,均有显著性差异(P<0.05或P<0.01)。竹盐对盐酸洛哌丁胺诱导的STC小鼠具有通便作用,可能通过调节SCF/c-kit和VIP/cAMP/PKA/AQP3信号通路达到缓解便秘效果,该研究为开发相关功能性食品提供理论基础和科学依据。To investigate the alleviating effect and mechanism of bamboo salt in mouse model of slow transit constipation(STC) induced by loperamide hydrochloride,the constipation model was established by inadministration of loperamide hydrochloride(10 mg/kg) for 7 consecutive days.Starting from the 8th day,loperamide hydrochloride was inadministration for 1 h,followed by inadministration of low,medium,and high doses(0.5,1.0,1.5 g/kg) of bamboo salt,2.5 mg/kg of mosapride citrate,and normal saline.On the 14th day after the end of intragastric administration,a number of defecation granules in 6hours were measured and the intestinal propulsion rate was calculated.The colon pathological changes were observed by hematoxylin-eosin staining,and the kits were used to determine serum levels of gastrin(Gas),substance-P(SP),and nitric oxide(NO).Western blot was used to determine the relative expression of c-kit proto-oncogene protein(c-kit),vasoactive intestinal peptide(VIP),protein kinase A(PKA),and aquaporin3(AQP3) in the colon.Use real-time quantitative PCR to determine the relative expression of stem cell factor(SCF) and cyclic adenosine monophosphate(cAMP) in colon tissue.Compared with the model group,the colonic muscle layer of STC mice in the bamboo salt medium-high dose group and the mosapride citrate group gradually thickened and goblet cells increased,which alleviated the mucosal damage caused by constipation.Compared with the model group,after the intervention of medium and high doses of bamboo salt and citric acid,intestinal propulsion rate and serum Gas and SP contents in STC mice were significantly increased,while NO contents were decreased,and the protein expression levels of c-kit,VIP,PKA and AQP3 in colon tissue and the relative mRNA expression of SCF and cAMP were promoted.There were significant differences(P<0.05 or P<0.01).Compared with the model group,the colonic muscle layer of STC mice in the bamboo salt medium-high dose group and the mosapride citrate group gradually thickened and goblet cells increased,which

关 键 词：竹盐 便秘 干细胞因子/c-kit原癌基因 血管活性肠肽/环磷酸腺苷/蛋白激酶A/水通道蛋白3 

分 类 号：R285.5[医药卫生—中药学]