柱前衍生化法分析木槿花多糖中单糖组成  

Analysis of Monosaccharide Constituent in Flos Hibiscus Polysaccharide by Precolumn Derivatization

作　　者：王静 孙盼盼 曹际云 WANG Jing;SUN Panpan;CAO Jiyun(Dezhou University,School of Life Sciences,Dezhou 253023)

机构地区：[1]德州学院生命科学学院,德州253023

出　　处：《食品工业》2024年第3期289-292,共4页The Food Industry

基　　金：德州学院大学生创新创业训练计划项目(901552);德州学院横向科研项目(HXKT2023292);山东省科技攻关重点研发项目(2019GSF108113);德州市科技计划项目(2022dzkj023)。

摘　　要：通过柱前衍生化-HPLC法对木槿花多糖中的单糖组成进行分析,并建立木糖、半乳糖、阿拉伯糖、葡萄糖、葡萄糖醛酸5种单糖的含量测定方法。对液相色谱条件、多糖水解条件、衍生化条件进行考察优化,通过比较单糖衍生物的峰面积,确定木槿花多糖水解产物单糖的含量测定方法。结果表明,液相色谱条件为C18色谱柱、30℃柱温、流动相0.1 moL/L乙酸铵溶液-乙腈(80+20,V/V)。水解条件为1 mol/L盐酸溶液、110℃水解温度、60 min水解时间。衍生化条件为70℃衍生化温度、90 min衍生化时间。该方法操作简单,重复性高,可用于测定木槿花多糖中单糖含量。Analyze the monosaccharide constituent of Hibiscus polysaccharide by pre-column derivatization-HPLC,and build a approach for the assay of xylose,galactose,arabinose,glucose and glucuronic acid.The condition of liquid chromatography,polysaccharide hydrolysis and derivatization was investigated and optimized.By comparing the peak areas of monosaccharide derivatives,the assay approach of monosaccharide content in polysaccharide hydrolysate of Hibiscus was determined.The results showed that HPLC conditions was that C18 column,column temperature of 30 ℃,and mobile phase of 0.1 mol/L ammonium acetate solution-acetonitrile(80+20,V/V).Hydrolysis condition was that hydrochloric acid solution of 1 mol/L,hydrolysis temperature of 110 ℃ and hydrolysis time of 60 min.Derivatization condition was that derivatization temperature of 70 ℃ and derivatization time of 90 min.The approach was simple and reproducible,and could be used to determine the monosaccharide content in polysaccharide of Hibiscus.

关 键 词：高效液相色谱法 柱前衍生化 木槿花多糖 单糖 含量测定 

分 类 号：O657.72[理学—分析化学] TS201.2[理学—化学]