N-acetylcysteine ameliorates erectile dysfunction in rats with hyperlipidemia by inhibiting oxidative stress and corpus cavernosum smooth muscle cells phenotypic modulation  

作　　者：Wei Ding Jun-Hong Fan Li-Ren Zhong Nan-Xiong Wang Lu-Hao Liu Hai-Bo Zhang Li Wang Ming-Qiang Wang Bing-Lin He An-Yang Wei 

机构地区：[1]Department of Urology,The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China [2]Department of Urology,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510000,China [3]Department of Urology,Nanfang Hospital,Southern Medical University,Guangzhou 510000,China [4]Department of Urology,Shenzhen Immigration Inspection General Station Hospital,Shenzhen 518000,China [5]Department of Organ Transplantation,The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510000,China [6]Department of Endocrinology,The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China

出　　处：《Asian Journal of Andrology》2024年第1期99-106,共8页亚洲男性学杂志（英文版）

基　　金：This work is supported by a grant from the National Natural Science Foundation of China(No.82060809);the Natural Science Foundation of Guangdong Province(No.2017A030313453 and No.2018A030313638);Guangzhou Basic and Applied Basic Research Foundation(No.202102020417).

摘　　要：Hyperlipidemia is a major risk factor for erectile dysfunction(ED).Oxidative stress and phenotypic modulation of corpus cavernosum smooth muscle cells(CcSMCs)are the key pathological factors of ED.N-acetylcysteine(NAC)can inhibit oxidative stress;however,whether NAc can alleviate pathological variations in the corpus cavernosum and promote erectile function recovery in hyperlipidemic rats remains unclear.A hyperlipidemia model was established using 27 eight-week-old male Sprague-Dawley(SD)rats fed a high-fat and high-cholesterol diet(hyperlipidemic rats,HR).In addition,9 male SD rats were fed a normal diet to serve as controls(NC).HR rats were divided into three groups:HR,HR+normal saline(NS),and HR+NAC(n=9 for each group;NS or NAc intraperitoneal injections were administered daily for 16 weeks).Subsequently,the lipid profiles,erectile function,oxidative stress,phenotypic modulation markers of ccsMCs,and tissue histology were analyzed.The experimental results revealed that erectile function was significantly impaired in the HR and HR+NS groups,but enhanced in the HR+NAC group.Abnormal lipid levels,over-activated oxidative stress,and multi-organ lesions observed in the HR and HR+NS groups were improved in the HR+NAC group.Moreover,the HR group showed significant phenotypic modulation of CCSMCs,which was also inhibited by NAC treatment.This report focuses on the therapeutic effect of NAc in restoring erectile function using a hyperlipidemic rat model by preventing CcSMC phenotypic modulationand attenuating oxidativestress.

关 键 词：erectile dysfunction HYPERLIPIDEMIA N-ACETYLCYSTEINE oxidative stress henotypic modulation 

分 类 号：R698.1[医药卫生—泌尿科学]