SsdchA is a novel secretory cellobiohydrolase driving pathogenicity in Sclerotinia sclerotiorum  

作　　者：Yangui Chen Yijuan Ding Siqi Zhao Nan Yang Zhaohui Wu Ping Zhang Hongmei Liao Mengquan Dong Yang Yu Huafang Wan Wei Qian 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China [2]Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China [3]Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,China [4]College of Plant Protection,Southwest University,Chongqing 400715,China

出　　处：《The Crop Journal》2024年第2期493-502,共10页作物学报（英文版）

基　　金：financially supported by the National Nature Science Foundation of China (32372077);the Project of Chongqing Science and Technology Commission (CSTB2023NSCQ-MSX0355);the Fundamental Research Funds for the Central Universities (SWU120075)。

摘　　要：The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum.

关 键 词：CELLOBIOHYDROLASE CELLULOSE PATHOGENICITY Sclerotinia sclerotiorum Secretory protein SsdchA 

分 类 号：S432[农业科学—植物病理学]