financially supported by the National Nature Science Foundation of China (32372077);the Project of Chongqing Science and Technology Commission (CSTB2023NSCQ-MSX0355);the Fundamental Research Funds for the Central Universities (SWU120075)。
The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. scleroti...
supported by grants from the National Key R&D Program of China(2021YFC2101300,2018YFA0900503);the National Natural Science Foundation of China(31970070);the Youth Interdisciplinary Science and Innovative Research Groups of Shandong University(2020QNQT006).
Cellulose degradation results from the synergistic effect of different enzymes,but which enzyme is involved in the initial stage of cellulose degradation is still not well understood.Cellobiohydrolase 2(CBH2)attached ...
Indigenous grasses have been effectively used to rehabilitate degraded African drylands. Despite their success, studies examining their effects on soil bioindicators such as microbial biomass carbon(C) and enzyme acti...
Aim: Lignocelluloytic enzymes are the largest class of hydrolase enzyme which utilizes the plant biomass to produce renewable sources. Hence practices for larger production of these enzymes at lower cost received much...
National Natural Science Foundation of China(#41171202,41125004).
Aims Nitrogen(N)addition could affect the rate of forest litter and soil organic matter decomposition by regulating extracellular enzyme activity(EEa).The impact of N addition on EEa may differ across different age st...
Supported by the National Natural Science Foundation of China (20976130 and 20806057), National Science and Technology Pillar Program of China (2007BAD42B02), Program for New Century Excellent Talents in University of Ministry of Education of China (No. NCET-08-0386), and the R&D program of Tianjin Binhai New Area (2010-BK17C004)..
The reactions of exo-cellulase (cellobiohydrolase, CBH) and endo-cellulase (endoglucanase, EG) were investigated by analyzing the insoluble residues of microcrystalline cellulose (MCC) and filter paper cellulose...
the National Natural Science Foundation of China(Grants No.30500007);Major State Basic Research Development Research Program of China(Grant No.2004CB719702);Scientific Research Reward Fund for ExcellentYoung and Middle-Aged Scientists in Shandong Province(Grants No.2005BS06004)
A homogeneous cellulose-binding module(CBM)of cellobiohydrolase I(CBHI)from Trichoderma pseudokoningii S-38 was obtained by the limited proteolysis with papain and a series of chromatographs filtration.Analysis of FT-...
the National Natural Science Foundation of China (Grant Nos. 30370013 and 30500007)
Conformational changes to 1,4-β-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl β-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrometry at the saturatio...
This work was supported by the grants from the National Natural Science Foundation of China (No. 30470052), the National Basic Research Program (973) of China (No. 2003CB716006 and 2004CB719702) and the Natural Science Research Foundation for the Doctoral Program of the Higher Education Ministry of China (No. 20040422042)
To improve heterologous gene expression in Trichoderma reesei, a set of optimal artificial cellobiohydrolase I gene (cbhl) promoters was obtained. The region from -677 to -724 with three potential glucose repressor ...
This work was supported by the grants from the Major State Basic Research Development Program (973) of China (N0. 2003CB716006 and 2004CB719702);the Natural Science Foundation of Shandong Province (No. L2003D01) ; the 0pen Foundation of State Key laboratory of Microbial Technology, Shandong University
A cellobiohydrolase 1 gene (cbhl) was cloned from Penicillium chrysogenum FS010 by a modified thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR). DNA sequencing shows that cbhl has an open reading ...