Box-Behnken响应面法优选平痤滴丸制备工艺及药效研究  

Optimization of preparation technology and study of pharmacodynamics on Pingcuo Dripping Pills by Box-Behnken response surface methodology

作　　者：张欣荣 董人华 王洁煊 程杰[1,2] ZHANG Xin-rong;DONG Ren-hua;WANG Jie-xuan(Pharmacy Department,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050011,China;Hebei IndustrialTechnology Institute for Traditional Chinese Medicine Preparation,Shijiazhuang 050011,China.;不详)

机构地区：[1]河北省中医院药学部,石家庄050011 [2]河北省中药制剂产业技术研究院,石家庄050011 [3]北京中医药大学中药学院,北京102488

出　　处：《中国处方药》2024年第4期6-12,共7页Journal of China Prescription Drug

基　　金：国家中医药管理局全国中药特色技术传承人才培训项目(20194828003);河北省中医院“国家中医临床研究基地建设”项目(冀中医院〔2019〕53号);河北省中医药管理局科研计划项目(2017050)。

摘　　要：目的在平痤散中药处方基础上,研制平痤滴丸,并对比研究平痤散和平痤滴丸对痤疮的作用。方法以综合评分为评价指标,考察因素为药物与基质质量比、药液温度、冷凝温度,采用Box-Behnken响应面法,对平痤滴丸的制备工艺进行优化;考察平痤散和平痤滴丸对小鼠激素水平、皮脂腺斑组织的影响,对耳肿胀的抗炎作用、对金色葡萄球菌和痤疮丙酸杆菌的抑制作用。结果最优滴丸制备工艺料液比为5∶1,药液温度为80℃,冷凝温度为0℃;平痤滴丸组体内激素水平睾酮(T)值、雌二醇(E2)值、T/E2值、小鼠耳肿胀程度及抑菌作用,均与阳性药组差异无统计学意义(P>0.05),且优于平痤散组。结论平痤滴丸组治疗痤疮效果显著,优于平痤散组,能够有效调节小鼠体内激素水平、减轻小鼠耳肿胀程度、抑制痤疮致病菌。Objective Based on the traditional Chinese medicine prescription of Pingcuo Powder,Pingcuo Drop Pills were developed,and the effects of Pingcuo Powder and Pingcuo Drop Pills on acne were compared and studied.Methods Using comprehensive scores as evaluation indicators,selecting drug to matrix mass ratio,liquid temperature,and condensation temperature as evaluation factors,the Box-Behnken response surface method was used to optimize the preparation process of Pingcuo Dripping Pills;To investigate the effects of Pingcuo Powder and Pingcuo Dropping Pills on hormone levels,sebaceous gland spot tissue in mice,anti-inflammatory effect on ear swelling,and inhibitory effect on Staphylococcus aureus and Propionibacterium acnes.Results The optimal preparation process for dripping pills has a material liquid ratio of 5∶1,a liquid temperature of 80℃,and a condensation temperature of 0℃;The T value,E2 value,T/E2 value,mouse ear swelling degree,and antibacterial effect of the Pingcuo Dropping Pill group were not significantly different from the positive drug group(P>0.05),and were superior to the Pingcuo Powder group.Conclusion The Pingcuo Dropping Pill group has a significant therapeutic effect on acne,which is superior to the Pingcuo Powder group.It can effectively regulate hormone levels in mice,reduce ear swelling in mice,and inhibit acne pathogenic bacteria.

关 键 词：滴丸 Box-Behnken响应面法 痤疮 耳肿胀 抑菌 

分 类 号：R283.6[医药卫生—中药学]