单细胞转录组测序及它在乳腺癌肿瘤微环境中的研究进展  

Single-cell RNA sequencing and its research progress in tumor microenvironment of breast cancer

作　　者：张光文 成晨 王世明[2] ZHANG Guangwen;CHENG Chen;WANG Shiming(The First Clinical College of Shanxi Medical University,Taiyuan 030001,P.R.China;Department of Breast Surgery,The First Hospital of Shanxi Medical University,Taiyuan 030001,P.R.China)

机构地区：[1]山西医科大学第一临床医学院,太原030001 [2]山西医科大学第一医院乳腺外科,太原030001

出　　处：《中国普外基础与临床杂志》2024年第4期495-501,共7页Chinese Journal of Bases and Clinics In General Surgery

基　　金：山西省基础研究计划项目面上项目(项目编号:202203021221250)。

摘　　要：目的了解单细胞RNA测序(single-cell RNA sequencing,scRNA-seq)技术及它在乳腺癌肿瘤微环境(tumor microenvironment,TME)中的研究进展,以期为乳腺癌研究与治疗提供新的思路与方向。方法对近年来国内外关于scRNA-seq技术的发展及它在乳腺癌TME中的相关研究文献进行综述。结果scRNA-seq是从细胞水平对mRNA进行高通量测序的一项量子技术,成为了组织样本量少时研究细胞异质性的有力工具,它可以在捕获罕见细胞类型的同时对乳腺癌TME的复杂结构进行精确的描绘。结论scRNA-seq经过几十年的发展,现已被广泛应用于肿瘤研究;乳腺癌是一类异质性极高的恶性肿瘤,将scRNA-seq应用于乳腺癌的研究中可以更好地了解其肿瘤异质性、TME,进而推进其个体化诊断和治疗的发展。Objective To understand the single-cell RNA sequencing(scRNA-seq)and its research progress in the tumor microenvironment(TME)of breast cancer,in order to provide new ideas and directions for the research and treatment of breast cancer.Method The development of scRNA-seq technology and its related research literature in breast cancer TME at home and abroad in recent years was reviewed.Results The scRNA-seq was a quantum technology in high-throughput sequencing of mRNA at the cellular level,and had become a powerful tool for studying cellular heterogeneity when tissue samples were fewer.While capturing rare cell types,it was expected to accurately describe the complex structure of the TME of breast cancer.Conclusions After decades of development,scRNA-seq has been widely used in tumor research.Breast cancer is a malignant tumor with high heterogeneity.The application of scRNA-seq in breast cancer research can better understand its tumor heterogeneity and TME,and then promote development of personalized diagnosis and treatment.

关 键 词：单细胞RNA测序 乳腺癌 肿瘤微环境 肿瘤异质性 

分 类 号：R737.9[医药卫生—肿瘤]