葛根渣中资源性化学成分分析及膳食纤维提取工艺研究  

Analysis of Resource Chemical Components and Extraction Technology of Dietary Fiber in Pueraria Root Residue

作　　者：曾慧婷 郑亚倩 戴迪 何小群 陈超[1,4] 王小青 虞金宝[1,3,4] 李晶 ZENG Hui-ting;ZHENG Ya-qian;DAI Di;HE Xiao-qun;CHEN Chao;WANG Xiao-qing;YU Jin-bao;LI Jing(Jiangxi Provincial Institute of Traditional Chinese Medicine,Nanchang 330046;Jiangxi University of Chinese Medicine,Nanchang 330004;Jiangxi Research Center for Protection and Development of Traditional Chinese Medicine Resources,Nanchang 330046;Jiangxi Quality Monitoring and Technology Service Center for Chinese Materia Medica Raw Materials,Nanchang 330046;Key Laboratory of Germplasm Selection and Breeding of Chinese Medicinal Materials,Nanchang,Jiangxi 330046)

机构地区：[1]江西省中医药研究院,南昌330046 [2]江西中医药大学,南昌330004 [3]江西省中药资源保护与开发利用工程研究中心,南昌330046 [4]江西省中药原料质量监测技术服务中心,南昌330046 [5]中药材种质选育繁育重点研究室江西,南昌330046

出　　处：《实用中西医结合临床》2024年第5期1-5,24,共6页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江西省中医药管理局科技计划项目(编号:2020B0401);江西省卫生健康委科技计划项目(编号:202311189);现代农业产业技术体系建设专项资金资助项目(编号:No.CARS-21)。

摘　　要：目的对葛根渣中资源性化学成分进行分析,并考察膳食纤维提取最佳工艺,以期为发现葛根渣资源价值和提升资源化利用效率提供参考。方法通过苯酚-硫酸法、酸碱消煮法分别对葛根渣中的多糖和粗纤维含量进行测定分析,基于单因素试验采用α-淀粉酶、糖化酶和木瓜蛋白酶进行膳食纤维的制备,并测定膳食纤维的膨胀力,确定最佳工艺参数。结果葛根渣中多糖含量为10.30%,粗纤维含量为15.58%;酶法制备膳食纤维的最佳工艺条件为:复合酶(α-淀粉酶、糖化酶)添加比例1:3、添加总量4%、酶解时间75 min、酶解温度65℃,蛋白酶添加总量为0.3%。结论葛根渣中多糖、粗纤维等资源性化学成分丰富,所建立的膳食纤维提取工艺可行,研究结果可为葛根渣的转化增效及粗细高值化利用模式提供科学依据。Objective Analyze the resource based chemical components in pueraria root residue and examine the optimal process for extracting dietary fiber,in order to provide reference for discovering the value of pueraria root residue resources and improving resource utilization efficiency.Methods The content of polysaccharides and crude fibers in pueraria root residue was determined and analyzed by using the phenol sulfuric acid method and the acid-base digestion method,respectively.In addition,based on a single factor experiment withα-amylase,saccharifying enzyme,and papain were used to prepare dietary fiber,and the swelling force of dietary fiber was measured to determine the optimal process parameters.Results The polysaccharide content in pueraria root residue was 10.30%,and the crude fiber content was 15.58%.The optimal process conditions for enzymatic preparation of dietary fiber were as follows the proportion of composite enzymes(α-amylase,saccharifying enzyme)added was 1:3,the total amount added was 4%,the enzymatic hydrolysis time was 75 minutes,the enzymatic hydrolysis temperature was 65℃,and the total amount of protease added was 0.3%.Conclusion The resource based chemical components such as polysaccharides and crude fibers in pueraria root residue are abundant,and the established dietary fiber extraction process is feasible.The research results can provide scientific basis for the transformation efficiency enhancement and high-value utilization mode of pueraria root residue.

关 键 词：葛根渣 化学成分 膳食纤维 资源价值 

分 类 号：R284.2[医药卫生—中药学]