嗜盐原油降解混合菌处理采出水特性研究  

Study on the Characteristics of Produced Water Treatment by Mixed Bacteria of Halophilic Crude Oil Degradation

作　　者：孙志超 刘力嘉 王明艳 王文娜[1] 宫禧茴 李召悦 刘春爽[1] Sun Zhichao;Liu Lijia;Wang Mingyan;Wang Wenna;Gong Xihui;Li Zhaoyue;Liu Chunshuang(College of Chemistry and Chemical Engineering,China University of Petroleum(East China),Qingdao 266400,China)

机构地区：[1]中国石油大学(华东)化学化工学院,山东青岛266580

出　　处：《山东化工》2024年第6期42-44,51,共4页Shandong Chemical Industry

摘　　要：针对高矿化度高硫化物的油田采出水的生物处理,从含油污泥、采出水等样品中经初筛、复筛获得嗜盐原油降解菌14株,选取降解率最高的4株进行复配,构建出嗜盐原油降解混合菌TG-1。16 SrDNA序列分析表明4株菌分别为不动杆菌(Acinetobacter);副蕈状芽胞杆菌(Bacillus paramycoides);芽孢杆菌(Bacillus sp.);肠杆菌(Enterobacter sp.)。考察了TG-1处理采出水性能,发现最适矿化度范围在30 g/L以下,最高耐受硫化物质量浓度可达150 mg/L,原油质量浓度不高于500 mg/L去除效果最好。将TG-1应用于生物接触氧化装置中,在pH值7~8、温度30℃左右、溶解氧4~5 mg/L、水力停留时间为4~12 h以及进水原油质量浓度为10~100 mg/L的条件下,出水原油质量浓度小于15 mg/L,满足克拉玛依油田注水水质标准A2。Aiming at the biological treatment of oilfield produced water with high salinity and high sulfide,14 strains of halophilic crude oil degrading bacteria were obtained from oily sludge,produced water and other samples through preliminary screening and rescreening.Four strains with the highest degradation rate were selected for compounding to construct a halophilic crude oil degrading mixed bacteria TG-1.16 SrDNA sequence analysis showed that the four strains were Acinetobacter;Bacillus paramycoides;Bacillus sp.;Enterobacter sp..The performance of TG-1 in the treatment of produced water was investigated.It was found that the optimum salinity range was below 30 g/L,the maximum tolerance sulfide concentration was 150 mg/L,and the degradation effect was the best when the crude oil concentration was not higher than 500 mg/L.The TG-1 was applied to the biological contact oxidation device.Under the conditions of pH 7~8,around temperature 30℃,dissolved oxygen 4~5 mg/L,hydraulic retention time 4~12 h and influent crude oil concentration 10~100 mg/L,the effluent crude oil concentration was less than 15 mg/L.It meets the Karamay oilfield water injection water quality standard A2.

关 键 词：原油降解 嗜盐菌 采出水处理 混合菌群 降解特性 

分 类 号：X172[环境科学与工程—环境科学]