基于复合微生物制剂的生物堆置处置研究  

Experimental Study on Biopile Disposal Based on Composite Microbial Formulations

作　　者：陈超 Chen Chao(Technical Testing Center,China Petroleum and Chemical Corporation Shengli Oilfield Branch,Dongying 257000,China)

机构地区：[1]中国石油化工股份有限公司胜利油田分公司技术检测中心,山东东营257000

出　　处：《山东化工》2024年第7期274-276,共3页Shandong Chemical Industry

摘　　要：为提升微生物修复含油土壤的效果,将XX地区含油土壤培养的土著菌剂作为修复菌剂,在室内开展生物堆小试试验,利用SPSS20.0软件做单因素方差分析,研究各项因素对提高菌剂修复的差异性和显著性;选择单因素条件的最优参数配合菌剂在XX地区对含油土壤进行现场修复。实验表明,在土壤石油烃含量为9 000 mg/m^(3)时,每500 mL土壤加菌剂0.5 g,菌棒和锯末含量为30%,含水率、温度分别保持在30%~40%、25~35℃范围内,pH值为6.8并适量添加无机盐(钙镁无机盐分别在130和250 mg/L)时的除油效果最好;利用培养的真菌-细菌强化修复石油污染技术应用于不同现场的时候是可行的,本研究为实践中利用微生物进行含油土壤修复提供科学的理论指导。In order to improve the effectiveness of microbial remediation of oily soil,indigenous microbial agents cultured in XX region were used as remediation agents.A small-scale biopile experiment was conducted indoors,and SPSS 20.0 software was used for one-way ANOVA to study the differences and significance of various factors in improving microbial remediation.Selecting the optimal parameters of single factor conditions combined with microbial agents for on-site remediation of oily soil in XX region.The experiment showed that the best oil removal effect was achieved when the soil petroleum hydrocarbon content was 9000 mg/m^(3),0.5 g of bacterial agent was added per 500 mL of soil,the content of bacterial rods and sawdust was 30%,the water content and temperature were kept within the range of 30%~40%,25~35℃,pH value was 6.8,and an appropriate amount of inorganic salt(calcium magnesium inorganic salt was 130 and 250 mg/L,respectively)was added.It is feasible to apply the enhanced remediation technology of petroleum pollution using cultivated fungi bacteria to different sites.This study provides scientific theoretical guidance for the use of microorganisms in the remediation of oily soil in practice.

关 键 词：石油污染土壤 生物制剂 生物刺激 生物强化 微生物修复 修复效果评价 

分 类 号：X172[环境科学与工程—环境科学] Q819[生物学—生物工程]