基于“气候因子-成分含量-抗氧化能力”评价不同产地葛根品质关系  

Evaluation of Relationship of Pueraria lobata Quality from Different Producing Areas Based on"Climate Factor-component Content-antioxidant Capacity"

作　　者：裴莉昕[1] 陈琳 王锴乐 纪宝玉[1,2,3,4] PEI Lixin;CHEN Lin;WANG Kaile;JI Baoyu(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Provincial Engineering and Technology Research Center for Ecological Planting of Genuine Medicinal Materials,Zhengzhou 450046,China;School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China;Collaborative Innovation Center of Research and Development on Whole Industry Chain of Yu-Yao,Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]河南省道地药材生态种植工程技术研究中心,郑州450046 [3]天津大学药物科学与技术学院,天津300072 [4]豫药全产业链研发河南省协同创新中心,郑州450046

出　　处：《中国实验方剂学杂志》2024年第10期140-148,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2017年度河南省高等学校青年骨干教师培养计划人选(2017GGJS082)。

摘　　要：目的:对葛根产地、气候因子、葛根总黄酮、葛根多糖含量及药用抗氧化活性等进行相关性分析,评价气候因子与葛根品质形成间的关系。方法:分别检测葛根总黄酮和多糖对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基的清除率,并分析葛根产地和气候因子及各组分含量之间的相关性。结果:葛根总黄酮ABTS^(+)清除率与海拔呈显著负相关(P<0.05),与年日照时数呈正相关(P<0.05);海拔与总黄酮含量呈正相关,年日照时数与总黄酮含量呈负相关;总黄酮含量与总黄酮ABTS^(+)清除率呈负相关;即产地海拔越低,年日照时数越长,则总黄酮含量越低,而总黄酮ABTS^(+)清除率越高。葛根多糖ABTS^(+)清除率与无霜期呈负相关(P<0.05),与7月均温呈负相关(P<0.01);无霜期与葛根多糖含量呈正相关,7月均温与葛根多糖含量呈正相关(P<0.05);葛根多糖含量与葛根多糖ABTS^(+)清除率呈负相关;即产地无霜期越短,7月均温越低,则葛根多糖含量越低,而葛根多糖ABTS^(+)清除率越高。7月均温均与葛根样品中总黄酮、多糖含量均呈相关性(P<0.05),7月均温越低,葛根总黄酮含量越高,葛根多糖含量越低,葛根样品的抗氧化活性越强。结论:葛根对ABTS^(+)的清除能力比DPPH^(+)强,各产地气候因子、含量及抗氧化能力之间具有显著相关性,对核心气候因子诱导葛根药用活性成分形成的内在规律展开进一步研究将为揭示葛根道地性形成机制及后续拟境调控葛根品质提供科学依据。Objective:The correlation of Pueraria lobata producing areas,climate factors,total flavonoids of P.lobata,polysaccharide content of P.lobata,and antioxidant activity of P.lobata for medicinal application was analyzed,and the relationship between climate factors and the formation of P.lobata quality was evaluated.Method:The scavenging rates of 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radicals by total flavonoids and polysaccharides of P.lobata were detected,and the correlation between the contents of each component and the information of producing areas and climate factors was analyzed.Result:The ABTS+scavenging rate by total flavonoids of P.lobata was negatively correlated with altitude(P<0.05)and positively correlated with annual sunshine hours(P<0.05).The altitude was positively correlated with the total flavonoid content,while the annual sunshine hours were negatively correlated with the total flavonoid content.There was a negative correlation between total flavonoid content and ABTS^(+)scavenging rate by total flavonoids.In other words,lower altitude and longer annual sunshine hours indicated lower total flavonoid content and higher ABTS^(+)scavenging rate by total flavonoids.The ABTS^(+)scavenging rate by polysaccharides of P.lobata was negatively correlated with the frost-free period(P<0.05)and the mean temperature in July(P<0.01).There was a positive correlation between the polysaccharide content of P.lobata and the frost-free period.The mean temperature in July was positively correlated with the polysaccharide content of P.lobata(P<0.05).The polysaccharide content of P.lobata was negatively correlated with the ABTS+scavenging rate by polysaccharides of P.lobata.In other words,a shorter frost-free period in the producing area and lower mean temperature in July indicated lower polysaccharide content of P.lobata and higher ABTS^(+)scavenging rate by polysaccharides of P.lobata.The mean temperature

关 键 词：产地 气候因子 葛根总黄酮 葛根多糖 抗氧化能力 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学] R289R287R22R2-031R33R24