激光诱导荧光检测鸡蛋抗生素残留的方法研究  

Study on Detection of Antibiotic Residues in Eggs by Laser-InducedFluorescence

作　　者：程文轩 张庆贤[1] 刘宇 邹立扣 CHENG Wen-xuan;ZHANG Qing-xian;LIU Yu;ZOU Li-kou(Key Laboratory of Geosciences and Nuclear Technology,Chengdu University of Technology,Chengdu 610059,China;College of Resources,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]成都理工大学,地学核技术四川省重点实验室,四川成都610059 [2]四川农业大学资源学院,四川成都611130

出　　处：《光谱学与光谱分析》2024年第5期1245-1254,共10页Spectroscopy and Spectral Analysis

基　　金：四川省科技计划项目(2021JDTD0018);四川省重大科技专项(2022ZDZX0017);国家自然科学基金项目(31671954)资助。

摘　　要：禽蛋产业中抗生素残留超标问题作为重要的食品安全问题之一,近些年来受到了广泛关注。目前对畜禽产品抗生素残留的检测方法主要采用微生物检测法、免疫分析法、高效液相色谱法等方法,但这些方法对于现场样品的快速检测还存在一定的不足。应用具有高灵敏度、高分辨率、速度快等特点的激光诱导荧光分析技术,开展鸡蛋中抗生素残留快速检测方法研究。样品以蛋清为溶剂,抗生素种类选用鸡蛋中可能残存的抗生素,包括环丙沙星、诺氟沙星、氧氟沙星、土霉素和庆大霉素,通过加入抗生素试剂模拟鸡蛋中抗生素的残留,测定了不同浓度下的抗生素荧光光谱。采用欧氏距离及概率密度的方法进行定性分析,定性分析的准确率在三倍标准差范围内达到了100%。在对已有的几种不同浓度样品进行检测分析后,建立了每种抗生素对应的c-S拟合曲线。结果表明,在有效检测范围内(环丙沙星:0.0001~1 mg·mL^(-1),诺氟沙星:0.0002~1 mg·mL^(-1),氧氟沙星:0.00005~1 mg·mL^(-1),土霉素:0.0001~0.2 mg·mL^(-1),庆大霉素:0.01~1 mg·mL^(-1)),可以根据荧光峰的净峰面积比较准确地计算抗生素含量。最后,计算了抗生素定量曲线的准确度和检出限,说明了该方法可以应用到鸡蛋中抗生素的残留量检测,但目前还存在检出限较高的问题,需要更进一步研究来降低检出限。In recent years,the issue of excessive antibiotic residues in the poultry egg industry has drawn considerable attention as a serious food safety concern.Currently,immunoassay,high-performance liquid chromatography,and microbial detection are the three primary techniques used to detect antibiotic residues in livestock and poultry products.When it comes to quick on-site sample testing,these technologies do have certain drawbacks.This study established a quick,accurate,and fast approach for detecting antibiotic residues in eggs using laser-induced fluorescence analysis.The antibiotics that might be found in chicken eggs were chosen for the samples,including ciprofloxacin,norfloxacin,ofloxacin,tetracycline,and gentamicin.The samples were created using egg white as the solvent.Antibiotic reagents were applied to imitate antibiotic residues in eggs,and the fluorescence spectra of antibiotics at various doses were examined.Euclidean distance and probability density approaches were used for the qualitative analysis,which had a 100%accuracy rate within three standard deviations.After examining samples at different concentrations,each antibiotic's calibration curves(c-S curves)were created.The results showed that within the effective detection range(ciprofloxacin:0.0001 to 1 mg·mL^(-1),norfloxacin:0.0002 to 1 mg·mL^(-1),ofloxacin:0.00005 to 1 mg·mL^(-1),tetracycline:0.0001 to 0.2 mg·mL^(-1),chloramphenicol:0.01 to 1 mg·mL^(-1)),the antibiotic content could be calculated more accurately based on the net peak area of the fluorescence spectra.Ultimately,the precision and detection bound of the antibiotic quantitative curve were computed,suggesting that the technique is applicable to detect antibiotic residues in eggs;however,the high detection limit remains an issue,necessitating additional investigation to lower the detection limit.

关 键 词：激光诱导荧光光谱法 抗生素残留 定性定量检测 

分 类 号：O433[机械工程—光学工程]