离体低温储存瘤块构建VX2肝癌模型效果研究  

The application of low temperature-storing tumor lumps in vitro in establishingVX2livercancer models:study of its modeling effect

作　　者：郭安然 陈宁 刘安娜 李玲[2] GUO Anran;CHEN Ning;LIU Anna;LI Ling(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning,Hubei Province 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院生物医学工程与医学影像学院,湖北咸宁437100

出　　处：《介入放射学杂志》2024年第3期269-274,共6页Journal of Interventional Radiology

基　　金：湖北省自然科学基金(2023AFB1077);湖北科技学院博士启动基金(BK202118)。

摘　　要：目的比较观察新鲜瘤块及不同时间低温冻存VX2肝癌瘤块经复苏后构建兔肝癌模型的效果。方法选取鱼肉状新鲜VX2瘤块,剔除周边坏死组织和肌肉后,-80℃下冰冻3、5和7个月。20只新西兰大白兔随机分为4组,A组予新鲜瘤块肝脏原位种植法构建VX2兔肝癌模型;B、C、D组分别给予-80℃冰冻3、5和7个月的瘤块,复苏后采用肝脏原位种植法构建兔肝癌模型。14 d后观察各组瘤兔肝脏的成瘤效果。通过免疫荧光观察肿瘤的增殖、凋亡及新生血管。结果A、B、C、D 4组的成瘤率为100%,但随着冻存时间的延长,5个月以后的瘤块活性差异性变大,肝脏肿瘤中心坏死面积增加。组织学检查发现,A、B两组模型TUNEL、Ki67、HIF1-α、VEGF、CD31表达无明显差异,而A、B组与C、D组相比,TUNEL、Ki67、HIF1-α、VEGF、CD31表达差异显著。结论-80℃下离体低温储存瘤块构建的兔VX2肝癌模型7个月内均可成瘤,5个月后瘤块间活性差异性变大,但整体来看可较好地保存瘤株活性,节省人力物力。Objective To compare the effect of using the fresh tumor lump and the recovered low temperature-storing VX2 tumor lump that have been frozen for different time to construct the rabbit HCC model. Methods Fish-like fresh VX2 tumor lumps were selected. After the peripheral necrotic tissue and muscle were removed, the tumor lumps were frozen at-80℃ for 3, 5 and 7 months. Twenty New Zealand white rabbits were randomly divided into 4 groups. The rabbits in group A(control group) received fresh liver tumor lump implantation to construct in-situ VX2 rabbit HCC models. The rabbits in groups B, C and D(experimental groups) received implantation of the recovered low temperature-storing VX2 tumor lump which had been frozen at-80℃ for 3, 5 and 7 months, respectively, to construct in-situ VX2 rabbit HCC models. Fourteen days after implantation, the modeling effect of tumor formation in each group was assessed. The proliferation,apoptosis of tumor cells and the angiogenesis were evaluated by immunofluorescence assay. Results The tumor formation rate of all group A, B, C and D was 100%. However, with the extension of cryopreservation time, the difference in tumor mass activity became larger after 5 months, and the necrotic area of liver tumor center became enlarged. Histological examination showed that there were no significant differences in the expressions of TUNEL, Ki67, HIF-α, VEGF and CD31 between group A and group B, while there were significant differences in the expressions of TUNEL, Ki67, HIF1-α, VEGF and CD31 between group A, B and group C, D. Conclusion The rabbit VX2 HCC model, which is constructed by implantation of recovered low temperature-storing VX2 tumor lump being frozen at-80℃ in vitro for a certain time, can be successfully established within 7 months. The difference in tumor mass activity between tumor lumps became larger after 5months. But on the whole, the constructed rabbit VX2 HCC model can better preserve the tumor strain activity. This modeling technique can save manpower and material resource

关 键 词：VX2肿瘤 肝癌 动物模型 

分 类 号：R735.7[医药卫生—肿瘤]