千家寨不同海拔野生大理茶代谢产物特征  

Characteristics of metabolites of wild Camellia talinensis at different altitudes in Qianjiazhai

作　　者：侯孟月 罗雯 程小毛[1] 肖云龙 黄晓霞[1] HOU Mengyue;LUO Wen;CHENG Xiaomao;XIAO Yunlong;HUANG Xiaoxia(College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University,Southwest Landscape Architecture Engineering Research Center of State Forestry and Grassland Administration,Kunming 650224,China;Zhenyuan Management and Protection Bureau of Ailao Mountain National Nature Reserve in Yunnan Province,Puer 666500,Yunnan,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业与草原局西南风景园林工程技术研究中心,昆明650224 [2]云南哀牢山国家级自然保护区镇沅管护局,云南普洱666500

出　　处：《生态学杂志》2024年第4期1092-1101,共10页Chinese Journal of Ecology

基　　金：云南省高层次人才“青年拔尖人才”(YNWR-QNBJ-2020-222)项目;国家自然科学地区基金项目(31760197);云南省农业联合面上项目(202101BD070001-041)资助。

摘　　要：以云南省千家寨自然保护区内4个海拔(2050、2200、2350、2500 m)的大理茶(Camellia talinensis)天然群体为研究对象,采用高效液相色谱(UPLC-MS/MS)比较不同海拔野生大理茶的代谢产物差异,探究大理茶对不同海拔的生理代谢响应与适应。结果表明:4个海拔的大理茶共检测到12类代谢物质,1013个代谢物,最多的是黄酮类物质(23.10%),其次是酚酸类物质(16.68%);筛选出430种差异代谢物,75种共有差异代谢物,其中,2050 m海拔的黄酮类物质更为丰富,2200 m海拔的氨基酸及其衍生物、核苷酸及其衍生物、生物碱类以及脂质类物质较多,而在2350 m及2500 m海拔上,酚酸类和有机酸类物质显著增多;此外,不同海拔下的差异代谢物显著富集在黄酮类生物合成途径、咖啡因代谢途径以及氨基酸代谢途径,且随海拔升高,黄酮类途径的关键代谢物含量呈现降-增-降的变化趋势,咖啡因和氨基酸途径的关键代谢物则呈现先增后降的变化。We compared the difference of metabolite components of wild ancient tea(Camellia talinensis)along an altitudinal gradient(2050,2200,2350,2500 m)of Qianjiazhai Reserve in Yunnan Province by UPLC-MS/MS,to explore the physiological and metabolic response and adaptation at different altitudes.The results showed that a total of 12 categories of metabolites and 1013 metabolites in C.talinensis were detected on the four altitudes.Flavonoids were the most common(23.10%),followed by phenolic acids(16.68%).A total of 430 differential metabolites were selected,with 75 shared differential metabolites.More flavonoids were enriched at 2050 m altitude,and more amino acids and their derivatives,nucleotides and their derivatives,alkaloids and lipid metabolites at 2200 m altitude.Phenolic acids and organic acids increased significantly at altitudes of 2350 m and 2500 m.In addition,different metabolites were significantly enriched in the flavonoid biosynthesis pathway,caffeine metabolism pathway and amino acid metabolism pathway at different altitudes.With increasing altitude,key metabolites of flavonoid pathway showed a down-up-down trend,while the key metabolites of caffeine and amino acid pathway showed a first increasing and then decreasing trend.

关 键 词：海拔 大理茶 高效液相色谱 代谢组学 代谢物 

分 类 号：S571.1[农业科学—茶叶生产加工]