饲粮中添加富含二十二碳六烯酸和月桂酸单甘酯的复合物对公猪精液品质的影响  

Effects of Dietary Docosahexaenoic Acid and Glycerol Monolaurate Enriched Complex on Semen Quality of Boars

作　　者：邬妍婷 杨志鹏 李亚梅 李豪 彭健[1,2,3] 王济华 魏宏逵 WU Yanting;YANG Zhipeng;LI Yamei;LI Hao;PENG Jian;WANG Jihua;WEI Hongkui(College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China;Frontiers Science Center for Animal Breeding and Sustainable Production,Wuhan 430070,China;Calid Biotech(Wuhan)Co.,Ltd.,Wuhan 430070,China)

机构地区：[1]华中农业大学动物科技学院,武汉430070 [2]生猪健康养殖部省共建协同创新中心,武汉430070 [3]动物育种与健康养殖前沿科学中心,武汉430070 [4]嘉利多生物技术(武汉)有限公司,武汉430070

出　　处：《动物营养学报》2024年第4期2262-2276,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业产业技术体系(CARS-36);湖北省重点研发计划(2022BBA0012)。

摘　　要：本试验旨在研究饲粮中添加富含二十二碳六烯酸(DHA)和月桂酸单甘酯(GML)的复合物对公猪精液品质的影响。选择48头19~24月龄杜洛克公猪,随机分成4组,每组12个重复,每个重复1头猪。对照组饲喂基础饲粮,试验1、2和3组分别在基础饲粮中添加0.18%、0.24%和0.30%富含DHA和GML的复合物。试验期16周。结果表明:1)试验2和3组的精子DHA、n-3多不饱和脂肪酸和多不饱和脂肪酸含量均极显著高于对照组(P<0.01)。2)在第16周时,试验1、2和3组的总精子数和有效精子数均显著高于对照组(P<0.05),精子畸形率均极显著低于对照组(P<0.01)。3)在第4、12和16周时,试验1、2和3组的精子膜完整性均极显著高于对照组(P<0.01);在第10周时,试验2和3组的精子膜完整性显著高于对照组(P<0.05)。4)试验1组的新鲜精液旋转运动精子比率显著低于对照组(P<0.05);在储存72 h后,试验1组旋转运动精子比率极显著低于对照组(P<0.01)。5)试验2组的血清丙二醛(MDA)含量极显著低于对照组和试验1、3组(P<0.01)。试验1和2组的新鲜精子超氧化物歧化酶(SOD)活性极显著高于对照组(P<0.01),试验2组的新鲜精浆SOD活性显著高于对照组(P<0.05)。在储存24 h后,试验1、2和3组的精子MDA含量均极显著低于对照组(P<0.01)。6)试验1和2组的血清白细胞介素-6(IL-6)含量极显著低于对照组(P<0.01)。试验1和2组的粪便二胺氧化酶(DAO)含量极显著低于对照组(P<0.01),试验1和2组的粪便钙卫蛋白含量显著低于对照组(P<0.05)。由此可见,富含DHA和GML的复合物通过影响精子脂肪酸组成、氧化应激和炎症状态等方面对公猪精液品质产生了积极影响。This experiment was conducted to study the effects of dietary docosahexaenoic acid(DHA)and glycerol monolaurate(GML)enriched complex on semen quality of boars.Forty-eight 19 to 24-month-old Duroc boars were randomly divided into 4 groups with 12 replicates per group and 1 pig per replicate.Pigs in the control group were fed a basal diet,and others in experimental groups 1,2 and 3 were fed the basal diet supplemented with 0.18%,0.24% and 0.30% DHA and GML enriched complex,respectively.The experiment lasted for 16 weeks.The results showed as follows:1)the contents of DHA,n-3 polyunsaturated fatty acids and polyunsaturated fatty acids in sperm of experimental groups 1,2 and 3 were significantly higher than those of the control group(P<0.01).2)On week 16,the total sperm count and effective sperm count of experimental groups 1,2 and 3 were significantly higher than those of the control group(P<0.05),and the sperm abnormal rate was significantly lower than that of the control group(P<0.01).3)On weeks 4,12 and 16,the sperm membrane integrity of experimental groups 1,2 and 3 was significantly higher than that of the control group(P<0.01);on week 10,the sperm membrane integrity of experimental groups 2 and 3 was significantly higher than that of the control group(P<0.05).4)The rotational motile sperm ratio in fresh semen of experimental group 1 was significantly lower than that of the control group(P<0.05);after stored 72 h,the rotational motile sperm ratio of experimental group 1 was significantly lower than that of the control group(P<0.01).5)The serum malondialdehyde(MDA)content of experimental group 2 was significantly lower than that of the control group and experimental groups 1 and 3(P<0.01).The fresh sperm superoxide dismutase(SOD)activity of experimental groups 1 and 2 was significantly higher than that of the control group(P<0.01),and the fresh seminal plasma SOD activity of experimental group 2 was significantly higher than that of the control group(P<0.05).After stored 24 h,the sperm MDA content of experimenta

关 键 词：公猪 二十二碳六烯酸 月桂酸单甘酯 精液品质 氧化应激 

分 类 号：S828[农业科学—畜牧学]