复合菌培养物对奶牛生产性能、非特异性免疫功能及抗氧化能力的影响  

Effects of Compound Bacteria Culture on Performance,Non-Specific Immune Function and Antioxidant Capacity of Dairy Cows

作　　者：李薛强 王岩 刘世雄 李松建[1,2] 杨岚 胡红莲[3] 高民 陈灰[1,2] 刘大程[1,2] LI Xueqiang;WANG Yan;LIU Shixiong;LI Songjian;YANG Lan;HU Honglian;GAO Min;CHEN Hui;LIU Dacheng(College of Veterinary,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases,Ministry of Agriculture and Rural Affairs,Hohhot 010018,China;Institute of Animal Nutrition and Feed,Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]农业农村部动物疾病临床诊疗技术重点实验室,呼和浩特010018 [3]内蒙古农牧业科学院动物营养与饲料研究所,呼和浩特010031

出　　处：《动物营养学报》2024年第4期2416-2424,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(32060774);国家现代农业产业技术体系资助(CARS36);内蒙古自治区科技计划技术(2022YFDZ0051);内蒙古自治区直属高校基本科研业务费项目(BR22-11-17)。

摘　　要：本试验旨在研究复合菌培养物对奶牛生产性能、非特异性免疫功能及抗氧化能力的影响。试验选择2胎次以及泌乳天数[(92.1±25.6)d]、产奶量[(36.8±4.6)kg/d]和乳蛋白质含量[(2.98±0.48)%]相近的健康荷斯坦奶牛40头,随机分为2组,每组20头。对照组饲喂基础饲粮,试验组在基础饲粮中添加0.23 kg/d的复合菌培养物。预试期7 d,正试期60 d。试验期间每日记录奶牛采食量和产奶量;每20 d采集1次牛奶测定乳成分;正试期开始和结束当天采集奶牛血液,测定乳成分以及免疫和抗氧化指标。结果表明:1)试验第41~60天,对照组奶牛平均日采食量比试验第1~20天降低了2.92%,而试验组提高了3.12%。试验第21~40天和第41~60天,试验组奶牛产奶量分别比对照组提高了2.17(P<0.01)和2.42 kg/d(P<0.05)。试验期间,2组乳蛋白质和非脂乳固体含量均无显著差异(P>0.05)。2)试验第60天,试验组奶牛血清溶菌酶(LZM)含量、酸性磷酸酶(ACP)活性、淋巴细胞增殖能力和中性粒细胞吞噬能力均显著高于对照组(P<0.05),并且试验组奶牛血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性分别比对照组显著提高了11.54%、22.09%和17.18%(P<0.05),血清丙二醛(MDA)含量比对照组显著降低了35.38%(P<0.05)。结果提示,复合菌培养物能够显著提高奶牛采食量和产奶量,改善奶牛非特异性免疫功能,增强机体抗氧化能力。This experiment was conducted to study the effects of compound bacteria culture on performance,non-specific immune function and antioxidant capacity of dairy cows.Forty healthy Holstein cows with two parities and similar lactation days[(92.1±25.6)d],milk yield[(36.8±4.6)kg/d]and milk protein content[(2.98±0.48)%]were randomly divided into 2 groups with 20 cows per group.Cows in the control group were fed a basal diet,and those in the experimental group were fed the basal diet supplemented with 0.23 kg/d compound bacteria culture.The pre-trial period lasted for 7 days and the experimental period lasted for 60 days.Feed intake and milk yield were recorded daily during the experiment.Milk was collected every 20 days to determine the milk composition,and blood was collected at the beginning and ending of the experiment to determine the immune and antioxidant indices.The results showed as follows:1)on days 41 to 60 of the experiment,the average daily feed intake of cows in the control group was decreased by 2.92% compared with days 1 to 20 of the experiment,while it was increased by 3.12% in the experimental group.On days 21 to 40 and 41 to 60,the milk yield of cows in the experimental group was increased by 2.17(P<0.01)and 2.42 kg/d(P<0.05)compared with that in the control group,respectively.During the experiment,there were no significant differences in milk protein and non-fat milk solid contents between 2 groups(P>0.05).2)On day 60,the serum lysozyme(LZM)content,acid phosphatase(ACP)activity,lymphocyte proliferation capacity and neutrophil phagocytosis capacity of cows in the experimental group were significantly higher than those in the control group(P<0.05),the total antioxidant capacity(T-AOC),activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in serum in the experimental group were significantly increased by 11.54%,22.09% and 17.18% compared with the control group(P<0.05),and the serum malondialdehyde content was significantly decreased by 35.38%compared with the control group(P<0.05),r

关 键 词：复合菌培养物 奶牛 生产性能 非特异性免疫功能 抗氧化能力 

分 类 号：S816[农业科学—饲料科学]