2'⁃岩藻糖基乳糖功能及其微生物生产菌种构建研究进展  

Research Progress on the Function of 2′‐Fucosyllactose and the Construction of Microbial Production Strains

作　　者：刘琳 赵藤 高俊哲 李俊众 孙雪 宗剑飞[3] 刘逸寒 李玉[1,2] 李庆刚[1,2] LIU Lin;ZHAO Teng;GAO Junzhe;LI Junzhong;SUN Xue;ZONG Jianfei;LIU Yihan;LI Yu;LI Qinggang(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education/Tianjin Key Laboratory of Industrial Microbiology/National Engineering Laboratory for Industrial Enzymes,Tianjin University of Science&Technology,Tianjin 300457,China;Shandong SynBio-Vision Technology Co.,Ltd.,Weifang 262500,Shandong,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津科技大学工业发酵微生物教育部重点实验室/天津市工业微生物重点实验室/工业酶国家工程实验室,天津300457 [3]山东合成远景生物科技有限公司,山东潍坊262500

出　　处：《食品研究与开发》2024年第8期207-216,共10页Food Research and Development

基　　金：2022年天津科技大学“大学生创新创业训练计划”项目(202210057133)。

摘　　要：2'⁃岩藻糖基乳糖(2'⁃fucosyllactose,2'⁃FL)在人乳寡糖中含量最高,占比可达30%。2'⁃FL能够有效促进婴儿大脑发育、提高婴儿免疫力,对青少年、成年人、老年人也大有裨益,已被广泛应用于婴幼儿配方奶粉、功能性食品饮料及医疗辅剂中。微生物合成2'⁃FL具有易实现大规模生产、可使用廉价原料作为底物等优势。目前2'⁃FL最主要的工业生产菌种为大肠杆菌,其他食品安全级菌株如酿酒酵母、枯草芽孢杆菌等也陆续被改造用来生产2'⁃FL,并已取得一定成效。该文对2'⁃FL作用于人体的机制、应用领域及2'⁃FL合成菌种构建现状等进行综述,并对未来发展趋势进行展望。2'⁃fucosyllactose(2'⁃FL),accounting for about 30%of human milk oligosaccharides,is the most abundant oligosaccharide in human milk.Recognized for its potential to promote infant brain development,en⁃hance infant immunity,and provide benefits across different age groups,2'⁃FL has been widely used in infant formula,functional food and beverage,and medical adjuvants.Microbial synthesis of 2'⁃FL has the advantages of easy large⁃scale production and using cheap raw materials as substrates.At present,the main industrial pro⁃duction strain of 2'⁃FL is Escherichia coli,while other food safety strains such as Saccharomyces cerevisiae and Bacillus subtilis have also been modified to produce 2'⁃FL,with promising results.This review comprehen⁃sively discusses the mechanisms by which 2'⁃FL impacts the human body,its diverse applications,the current state of microbial strain engineering for 2'⁃FL synthesis,and offers insights into future trends in this field.

关 键 词：2'⁃岩藻糖基乳糖 人乳寡糖 代谢工程 工业微生物 菌种构建 

分 类 号：TS201.3[轻工技术与工程—食品科学]