Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo  

作　　者：Fairouz Sioud Aida Lahmer Mouna Selmi Fadwa Chaabane Leila Chekir-Ghedira 

机构地区：[1]Research Unit Bioactive Natural Products and Biotechnology UR17ES49,Faculty of Dental Medicine of Monastir,University of Monastir,Avicenne Street,5000 Monastir,Tunisia [2]Laboratory of Pharmacognosy,Faculty of Pharmacy,University of Monastir,Avicenne Street,5000 Monastir,Tunisia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第4期154-161,共8页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo.Methods:The effects of the methanolic extract of Ephedra alata on the viability,migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,Transwell assay,and annexin V-FITC staining assay,respectively.Histological examination was also carried out.Moreover,a murine breast cancer model was established to evaluate the inhibitory effect of the extract.Biochemical parameters including hepatic and non-hepatic enzymes,malondialdehyde,and glutathione were investigated.Results:The methanolic extract of Ephedra alata showed a strong anti-proliferative and anti-migratory activity against 4T1 cells in a dose-dependent manner.It also induced apoptosis in 4T1 cells.In an in vivo mouse model,the extract markedly inhibited tumor growth,reduced malondialdehyde,and hepatic and non-hepatic enzymes as well as increased glutathione level.Conclusions:The methanolic extract of Ephedra alata inhibits breast cancer in vitro and in vivo,which may be a promising anticancer agent.

关 键 词：EPHEDRA Breast cancer Apoptosis ANTIOXIDANT MIGRATION 

分 类 号：R965[医药卫生—药理学]