LncRNA MIAT靶向miR-206促进食管鳞状细胞癌细胞的增殖、凋亡、迁移和侵袭的作用研究  

LncRNA MIAT Promotes the Proliferation,Apoptosis,Migration and Invasion of Esophageal Squamous Cell Carcinoma Cells by Targeting miR-206

作　　者：王海明 赵高峰[1] Wang Haiming;Zhao Gaofeng(Department of Lung Transplantation Surgery·Thoracic Surgery III,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院肺移植外科·胸外科三,郑州450000

出　　处：《成都医学院学报》2024年第2期242-247,共6页Journal of Chengdu Medical College

基　　金：郑州市协同创新专项项目(No:XTCX2023009)。

摘　　要：目的 探讨长链非编码RNA(LncRNA)MIAT靶向微小RNA(miR)-206促进食管鳞状细胞癌细胞的增殖、凋亡、迁移和侵袭的作用。方法 将食管鳞状细胞癌(ESCC)细胞分为ctrl组(正常培养的ESCC细胞)、si-NC组、si-MIAT组、si-MIAT+miR-NC组和si-MIAT+miR-206 inhibitor组。实时qRT-PCR检测各组细胞MIAT、miR-206表达;CCK-8法检测细胞增殖情况;划痕实验检测细胞迁移能力;流式细胞术分析细胞凋亡情况;蛋白质印迹技术检测PCNA、MMP-9蛋白表达;双荧光素酶报告基因实验验证MIAT和miR-206的关系。结果 与ctrl组、si-NC组比较,si-MIAT组ESCC细胞中miR-206表达、细胞凋亡率升高,MIAT、OD_(450)值(24、48、72 h)、划痕愈合率、PCNA和MMP9蛋白表达降低(P<0.05);干扰LncRNA MIAT表达能降低ESCC细胞增殖、迁移、侵袭能力,提高细胞凋亡能力,MIAT靶向调控miR-206表达。结论 干扰LncRNA MIAT表达能够阻滞ESCC细胞增殖、迁移、侵袭,并促进ESCC细胞凋亡。Objective To investigate the role of long non-coding RNA(LncRNA)myocardial infarction associated transcript(MIAT)in promoting the proliferation,apoptosis,migration and invasion of esophageal squamous cell carcinoma(ESCC)cells by targeting microRNA-206(miR-206).Methods ESCC cells were grouped into ctrl group(normal ESCC cells),si-NC group,si-MIAT group,si-MIAT+miR-NC group,and si-MIAT+miR-206 inhibitor group.The expression of MIAT and miR-206 was detected by real-time qRT-PCR;Cell proliferation was detected by CCK-8 method;Cell migration was detected by scratch test;Cell apoptosis was analyzed by flow cytometry;Western blot was applied to detect the expression of PCNA and MMP-9 proteins;Double luciferase reporter gene experiment was used to verify the relationship between MIAT and miR-206.Results Compared with ctrl group and si-NC group,the miR-206 expressions and apoptosis rate of ESCC cells in si-MIAT group increased,the MIAT,OD_(450) value(24h,48h,72h),scratch healing rate,PCNA and MMP9 protein expressions decreased(P<0.05).Interference with LncRNA MIAT expression reduced the proliferation,migration and invasion abilities and improved the apoptosis ability of ESCC cells.And MIAT was able to target the expression of miR-206.Conclusion Interference with LncRNA MIAT expression can block the proliferation,migration and invasion of ESCC cells,and promote the apoptosis of ESCC cells.

关 键 词：LncRNA MIAT 微小RNA-206 食管鳞状细胞癌 增殖 凋亡 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]