肝癌中Tenascin-C表达及其对肝癌Hep3B、SMMC-7721细胞侵袭转移影响的实验研究  

Experimental study on expression of Tenascin-C in hepatocellular carcinoma and its effect on invasion and metastasis of hepatocellular carcinoma Hep3B and SMMC-7721 cells

作　　者：高秀梅 朱萌 卢新兰 和水祥[3] GAO Xiumei;ZHU Meng;LU Xinlan;HE Shuixiang(The Second Department of Hepatology,Xi′an Eighth Hospital,Xi′an 710061,China)

机构地区：[1]西安市第八医院肝病二科,西安710061 [2]宁夏医科大学基础医学院,750004 [3]西安交通大学第一附属医院消化内科,710061

出　　处：《临床肿瘤学杂志》2024年第3期224-228,共5页Chinese Clinical Oncology

摘　　要：目的探讨Tenascin-C在人肝细胞癌中的表达,分析其表达水平与肝癌细胞侵袭转移的关系。方法体外培养人永生化肝细胞L-02、HL-7702和不同转移潜能人肝癌细胞Hep3B、HepG2、SMMC-7721、Bel-7402、HHCC、MHCC-97H、MHCC-97L,实时荧光定量PCR(qPCR)和免疫印迹法检测Tenascin-C的表达,Transwell检测人肝癌细胞的迁移和侵袭能力。构建Tenascin-C小干扰RNA,Transwell检测肝癌Hep3B、SMMC-7721细胞迁移和侵袭能力的变化。分析Tenascin-C表达与肝癌细胞侵袭转移的关系。结果与永生化肝细胞比较,人肝癌细胞中Tenascin-C mRNA和蛋白表达量均增高,差异均具有统计学意义(P=0.001);不同人肝癌细胞转移能力具有差异,以Hep3B最高,其次为SMMC-7721、Bel-7402,HepG2,MHCC97-L最低。沉默Tenascin-C后,与阴性对照组比较,肝癌Hep3B、SMMC-7721细胞迁移和侵袭能力均降低,差异具有统计学意义(P=0.001)。相关性分析显示,Tenascin-C表达与肝癌细胞迁移、侵袭数目均呈正相关(P=0.001)。结论Tenascin-C在人肝癌中的高表达可能参与促进癌细胞的侵袭转移,沉默Tenascin-C能够有效抑制肝癌Hep3B、SMMC-7721细胞转移能力,有望作为肝癌进展的重要生物学指标。Objective To investigate the expression of Tenascin-C in human hepatocellular carcinoma(HCC)and analyze the relationship between the expression level and invasion and metastasis of HCC cells.Methods Human immortable hepatocytes L-02,HL-7702 and human hepatocellular carcinoma cells Hep3B,HepG2,SMMC-7721,Bel-7402,HHCC,MHCC-97H,MHCC-97L were cultured in vitro.Real-time fluorescence quantitative PCR(qPCR)and Western blotting were used to detect the expression of Tenascin-C,and Transwell was used to detect the migration and invasion ability of human hepatoma cells.Tenascin-C small interfering RNA was constructed and Transwell was used to detect the changes of migration and invasion ability of hepatoma Hep3B and SMMC-7721 cells.The correlation between the expression of Tenascin-C and invasion and metastasis of hepatocellular carcinoma cells was analyzed.Results Compared with immortalized hepatocytes,the expression levels of Tenascin-C mRNA and protein in human hepatocellular carcinoma cells were increased,with statistical significance(P=0.001).The metastasis ability of human hepatocellular carcinoma cells is different,Hep3B is the highest,followed by SMMC-7721,Bel-7402,HepG2,MHCC97-L is the lowest.After Tenascin-C was silenced,hepatoma Hep3B and SMMC-7721 cells had decreased migration and invasion ability compared with the negative control group,and the difference was statistically significant(P=0.001).Correlation analysis showed that Tenascin-C was positively correlated with the number of liver cancer cell migration and invasion(P=0.001).Conclusion The high expression of Tenascin-C in human hepatocarcinoma may be involved in promoting the invasion and metastasis of cancer cells.Silencing Tenascin-C can effectively inhibit the metastasis ability of hepatocarcinoma Hep3B and SMMC-7721 cells,which is expected to be an important biological indicator for the progression of hepatocarcinoma.

关 键 词：肝细胞癌 侵袭 转移 TENASCIN-C 

分 类 号：R735.7[医药卫生—肿瘤]