乳腺癌组织中LRP2表达及临床意义  

Expression of LRP2 in breast cancer and its clinical significance

作　　者：孔秋梅[1] 王帅兵 郭鹏[1] KONG Qiumei;WANG Shuaibing;Guo Peng(Department of Oncology,Langfang People′s Hospital,Langfang 065000,China)

机构地区：[1]廊坊市人民医院肿瘤科,河北廊坊065000 [2]河北中石油中心医院肿瘤科,065000

出　　处：《临床肿瘤学杂志》2024年第3期229-233,共5页Chinese Clinical Oncology

基　　金：河北省省级科技计划自筹经费资助项目(182777147)。

摘　　要：目的探讨乳腺癌组织中LRP2表达与淋巴结转移及预后的影响。方法采用实时荧光定量PCR(qPCR)和免疫组化染色法检测LRP2基因和蛋白表达。分析LRP2表达与乳腺癌临床病理特征的关系,采用Kaplan-Meier法绘制生存曲线,生存差异行Log-rank检验。结果从GSE42568数据集筛出LRP2是乳腺癌中的差异表达基因。免疫组化染色结果显示,乳腺癌组织中LRP2染色评分低于癌旁正常组织(P<0.05);有淋巴结转移组(LMP)LRP2染色评分低于无淋巴结转移组(LMN),差异有统计学意义(P<0.05)。qPCR检测结果显示,LMP组LRP2基因表达水平显著低于LMN组,差异有统计学意义(P<0.05)。LRP2蛋白表达与T分期、淋巴结转移以及TNM分期有关(P<0.05),与年龄、分化程度无关(P>0.05)。分析TIMER 2.0数据库中乳腺癌患者的RNA测序数据发现LRP2低表达患者的总生存率显著低于LRP2高表达的患者(HR=0.75,P<0.05)。结论LRP2在乳腺癌组织中的低表达,其低表达与肿瘤淋巴结转移、预后不良有关。Objective To investigate the effect of LRP2 expression on lymph node metastasis and prognosis in breast cancer.Methods Real-time fluorescence quantitative PCR(qPCR)and immunohistochemical staining were used to detect the expression of LRP2 gene and protein.The relationship between LRP2 expression and clinicopathological features of breast cancer was analyzed.Kaplan-Meier method was used to draw the survival curve,and the survival difference was tested by Log-rank.Results LRP2 was screened as a differential gene in breast cancer expression from GSE42568 data set.Immunohistochemical staining showed that the LRP2 staining score in breast cancer tissues was lower than that in adjacent normal tissues(P<0.05).The LRP2 staining score in the group with lymph node metastasis(LMP)was lower than that in the group without lymph node metastasis(LMN),and the difference was statistically significant(P<0.05).The result of qPCR showed that the expression level of LRP2 gene in LMP group was significantly lower than that in LMN group,and the difference was statistically significant(P<0.05).The expression of LRP2 protein was correlated with T stage,lymph node metastasis and TNM stage(P<0.05),but not with age and differentiation degree(P>0.05).Analysis of RNA sequencing data of breast cancer patients in the TIMER 2.0 database showed that the overall survival rate of patients with low LRP2 expression was significantly lower than that of patients with high LRP2 expression(HR=0.75,P<0.05).Conclusion The low expression of LRP2 in breast cancer tissues is associated with lymph node metastasis and poor prognosis.

关 键 词：乳腺癌 低密度脂蛋白相关受体2(LRP2) 淋巴结转移 预后 标志物 

分 类 号：R739.7[医药卫生—肿瘤]