酶法辅助提取生姜姜辣素及其对山茶油抗氧化作用研究  

Enzyme-Assisted Extraction of Ginerols from Ginger ( Zingiber officinale Roscoe)and Its Antioxidant Effects on Camellia Oil

作　　者：沈玉平 刘玉 谢依帆 何春兰 张祖姣 Shen Yuping;Liu Yu;Xie Yifan;He Chunlan;Zhang Zujiao(Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area,Hunan Provincial Engineering Research Center for Ginkgo Biloba,College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou,425199)

机构地区：[1]湖南南岭地区植物资源研究开发湖南省工程研究中心,湖南省银杏工程技术研究中心,湖南科技学院化学与生物工程学院,永州425199

出　　处：《中国粮油学报》2024年第3期124-132,共9页Journal of the Chinese Cereals and Oils Association

基　　金：湖南省自然科学基金项目(2022JJ30273,2020JJ5204);湖南省教育厅科学研究项目(22B0804)。

摘　　要：油脂氧化是山茶油变质和品质降低的主要原因。姜辣素具有良好的抗氧化活性,提取的关键在于破坏生姜细胞结构。采用单因素实验和正交实验研究了酶用量、酶解温度、酶解pH和酶解时间对姜辣素提取率的影响,并采用Schaal烘箱法研究了姜辣素提取物对山茶油的抗氧化作用。结果显示,淀粉酶和纤维素酶复合酶处理对姜辣素提取具有协同增效作用,最佳配比为3∶2;单因素实验和正交实验优化后的最佳工艺条件为:酶用量15 mg/g干姜粉,酶解温度55℃,酶解pH为5.5,酶解时间1.25 h。在此条件下,姜辣素提取率为(4.75±0.07)%。姜辣素提取物对山茶油具有较好的抗氧化作用,其效果随提取物姜辣素浓度提高而增强,但与BHT仍有一定差距;8-姜酚、10-姜酚和6-姜烯酚与姜辣素提取物复配,均对山茶油抗氧化具有协同增效作用,其抗氧化效果接近BHT。Lipid oxidation is the major cause of deterioration and quality decrease of camellia oil.Gingerol has been reported to possess good antioxidant properties,and the key to gingerols extraction is the destruction of the structure of ginger cells.In this study,the effects of enzyme dosage,reaction temperature,reaction pH value and reaction time on the extraction yield of gingerols were studied by single factor and orthogonal experiments.The antioxidant effects of crude extract of gingerols on camellia oil were studied by Schaal oven method.The results showed that pre-treatment with combination of amylase and cellulose had a synergistic effect on gingerols extraction yield,and the optimum ratio was 3∶2.The optimum conditions were as follows:enzyme dosage,reaction temperature,reaction pH value and reaction time were 15 mg/g dried ginger powder,55℃,pH 5.5 and 1.25 h,respectively.Under these conditions,the gingerols extraction yield increased to(4.75±0.07)%.The crude extract of gingerols had good antioxidant effects on camellia oil,and the antioxidant effects were enhanced with the increase of gingerol concentration of the extract,but there was still a certain gap with BHT.8-gingerol,10-gingerol and 6-shogaol all had synergistic effects on the antioxidant effects of the crud extract of gingerols on camellia oil,and each of the antioxidant effects was close to BHT.

关 键 词：姜辣素 山茶油 提取 抗氧化 正交实验 

分 类 号：TS22[轻工技术与工程—粮食、油脂及植物蛋白工程]