苦荞FtERF基因克隆、生物信息学及其表达分析  

Cloning,Bioinformatics and Expression Analysis of FtERF Gene in Fagopyrum tataricum

作　　者：张俊 蔡苏云 徐子豪 侯蕾 贺润丽 尹桂芳[2] 王莉花[2] 王艳青[2] 卢文洁[2] 孙道旺[2] Zhang Jun;Cai Suyun;Xu Zihao;Hou Lei;He Runli;Yin Guifang;Wang Lihua;Wang Yanqing;Lu Wenjie;Sun Daowang(College of Traditional Chinese Medicine and Food Engineering,Shanxi University of Traditional Chinese Medicine,Taiyuan 030619,Shanxi,China;Biotechnology and Genetic Germplasm Resources Research Institute,Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming 650201,Yunnan,China)

机构地区：[1]山西中医药大学中药与食品工程学院,山西太原030619 [2]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,云南昆明650201

出　　处：《作物杂志》2024年第2期23-29,共7页Crops

基　　金：国家自然科学基金(31460379);财政部和农业农村部国家现代农业产业技术体系(CARS-07-C-2);山西省重点研发计划(201803D221012-6);山西省自然科学研究面上项目(20210302123231)。

摘　　要：乙烯响应因子对植物发育和细胞代谢具有重要作用。通过RT-PCR从苦荞中克隆出ERF基因,对其进行生物信息学分析和差异表达分析。结果表明,FtERF基因开放阅读框长度为729 bp,编码了243个氨基酸,编码的蛋白分子量26.08 k D,等电点9.22,属于亲水性蛋白。FtERF不含有信号肽和跨膜螺旋结构,亚细胞定位在细胞核内,蛋白质二级结构和三级结构高度相似。FtERF含有抑制基序DLNxxP,系统进化表明FtERF和ERF4关系较近。经荧光定量表达发现,厚壳苦荞不同部位表达均高于薄壳苦荞,苦荞发育成熟期表达高于非成熟期。Ethylene response factor plays an important role in plant development and cell metabolism.ERF gene was cloned from Tartary buckwheat by RT-PCR,and its bioinformatics and differential expression were analyzed.The results showed that the length of open reading frame of FtERF gene was 729 bp,encoding 243 amino acids,molecular weight of 26.08 kD,isoelectric point of 9.22,belonging to the hydrophilic protein.FtERF didn't contain signal peptides and transmembrane helical structure,and was located in the nucleus of the cell.The secondary and tertiary structures of the proteins were highly similar.FtERF contained the inhibitory motif DLNxxP,and phylogenetic evolution suggested that FtERF and ERF4 were closely related.The results showed that the expression of Tartary buckwheat in different parts of thick-shell were higher than those in thin-shell Tartary buckwheat,and the expression of Tartary buckwheat in mature period was higher than that in immature period.

关 键 词：苦荞 乙烯响应因子 生物信息学 表达分析 

分 类 号：S517[农业科学—作物学]