激光共聚焦显微镜应用于本科生的实验教学设计  

Experimental teaching design of confocal laser scanning microscope for undergraduates

作　　者：崔玉晓 陈翠红[1] 卢媛[1] 杨丽萍[1] 王雁南[1] 杨楠 CUI Yuxiao;CHEN Cuihong;LU Yuan;YANG Liping;WANG Yannan;YANG Nan(College of Environmental Science and Engineering,Nankai University,Tianjin 300350,China)

机构地区：[1]南开大学环境科学与工程学院,天津300350

出　　处：《实验室科学》2024年第2期28-32,共5页Laboratory Science

基　　金：天津市科学技术普及项目(项目编号:23KPXMRC00150,22KPHDRC00370);南开大学实验课程教学改革项目(项目编号:23NKSYKF02,23NKSYKF04,23NKSYKF06);南开大学实验教学改革项目(项目编号:21NKZZYQ09)。

摘　　要：激光共聚焦显微镜等大型仪器的实验教学是实现“新工科”建设的重要推动力,然而目前针对本科生的大型仪器教学却未得到有效普及。通过选取适合于本科生实践操作的微生物荧光染色观察实验以及基于“雨课堂”进行线上线下教学,发挥激光共聚焦显微镜可视化强的特点,激发本科生的求知欲望和探索热情,增强学生的主观能动性,促进以教师为主导向以学生为中心的教学模式转变。实践教学表明,易于操作、可视化强的实验方案有助于学生理解和掌握激光共聚焦显微镜相关的基础知识和未来应用,拓宽学生的知识面和科研视野,提高本科生的实践创新能力。The experimental teaching course of large-scale instruments such as confocal laser scanning microscope is an important driving force for the construction of“Emerging Engineering Education”.However,the teaching course of large-scale instruments for undergraduates has not been effectively popularized until now.This teaching design investigated the course reforming of confocal laser scanning microscope for undergraduates by utilizing the fluorescence staining experiments of bacteria that were suitable for the practical operation of undergraduates.The visualization features of confocal laser scan-ning confocal microscope may inspire undergraduatesdesire and exploration enthusiasm,enhance the subjective initiative of the students,and facilitate the transformation of teacher-centered teaching mode to student-centered teaching mode.Practical teaching indicated that the easy-to-operate and highly visualized protocols will help students understand the basic knowledge and future applications of confo-cal laser scanning microscopy,broaden the view and scope of knowledge,and improve the practical in-novation ability of undergraduates.

关 键 词：激光共聚焦显微镜 实验教学 雨课堂 课程改革 大型仪器 

分 类 号：G642.0[文化科学—高等教育学]