铁蓄积大鼠食管黏膜组织差异表达基因的筛选及生物学功能分析  

Screening and biological function analysis of differentially expressed genes in esophageal mucosa of rats with iron accumulation

作　　者：刘国红[1] 任雨轩 邵谦毅 梁硕 王丽萍[1] LIU Guohong;REN Yuxuan;SHAO Qianyi;LIANG Shuo;WANG Liping(School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001,China;不详)

机构地区：[1]郑州大学基础医学院组织学与胚胎学系,郑州450001 [2]郑州大学基础医学院

出　　处：《山东医药》2024年第12期37-41,共5页Shandong Medical Journal

摘　　要：目的筛选铁蓄积大鼠食管黏膜组织差异表达基因,并分析差异表达基因的生物学功能。方法12只6周龄雄性SD大鼠随机分为铁蓄积组和对照组,每组6只,铁蓄积组隔天腹腔注射蔗糖铁溶液制备铁蓄积模型,对照组注射等量生理盐水,14周后取血和食管、肝等组织,应用血清学、组织学方法鉴定铁蓄积造模是否成功;剥离两组食管黏膜组织,采用转录组测序技术筛选差异表达基因,并对差异表达基因进行基因本体(GO)功能富集分析及真核生物蛋白相邻类的聚簇(KOG)分类富集分析。结果筛选出9个差异表达基因,5个上调基因包括昼夜相关转录抑制因子(Ciart)、液泡蛋白质分选因子25(Vps25)、甲状腺激素应答蛋白(Thrsp)、UDP-N-乙酰氨基葡萄糖焦磷酸化酶1(Uap1)、血清解整合素—金属蛋白酶33(Adam33),4个下调基因包括过氧化物酶基因(Pxdn)、硫酸乙酰肝素蛋白多糖基因2(Hspg2)、中心体相关蛋白2(Cep2)、G蛋白信号转导调节因子4(Rgs4)。GO功能富集分析显示,上调基因的生物过程(BP)集中在节律过程、代谢过程、行为、生物过程调节、特定位置运动、生物过程的负调控、生物调节、定位、细胞过程、多细胞生物过程;细胞组成(CC)主要集中在膜封闭腔、细胞器部分、细胞器、膜、细胞外区域部分、膜部分、细胞部分、细胞;分子功能(MF)主要集中在结合、结构分子活性、催化活性。下调基因的BP主要集中在解毒、刺激反应、细胞组成或生物形成、信号、生物过程的负调控、生物过程的正调节、发育过程、细胞过程、生物过程调节、生物调节、代谢过程;CC主要集中在细胞外基质、细胞外基质组成、细胞外区域部分、细胞外区域、细胞器、膜、细胞部分、细胞;MF主要集中在酶调节活性、抗氧化活性、分子功能调节剂、受体调节活性、结构分子活性、结合、催化活性。KOG分类富集分析显示Objective To screen the differentially expressed genes(DEGs)in the esophageal mucosa of rats with iron accumulation and to analyze the biological function of DEGs.Methods Twelve 6-week-old male Sprague-Dawley rats were divided into the iron accumulation group(n=6)and control group(n=6).Rats in the iron accumulation group received bi-daily injections of sucrose iron solution,while rats in the control group received the equal volume of normal saline.After 14 weeks,the blood,esophagus and other organs were collected,and histological and serological techniques were employed to confirm weather modeling was successful or not.Esophageal mucosa was dissected for transcriptome sequencing,and DEGs were screened and annotated.The functional annotations of DEGs were conducted by Gene Ontology(GO)and the classification annotations of DEGs were conducted by clusters of orthologous groups for eukaryotic complete genomes(KOG).Results A total of 9 DEGs were screened;5 significantly up-regulated genes included circadian-asso-ciated repressor of transcription(Ciart),vacuolar protein sorting 25 homolog(Vps25),thyroid hormone-responsive pro-tein(Thrsp),UDP-N-acetylglucosamine pyrophosphorylase 1(Uap1),and ADAM metallopeptidase Domain 33(Ad-am33);4 significantly down-regulated genes included peroxidasin(Pxdn),heparan sulfate proteoglycan 2(Hspg2),cen-trosomal protein 2(Cep2)and regulator of G-protein signaling 4(Rgs4).GO enrichment analysis showed that the biologi-cal process(BP)of up-regulated genes was mainly concentrated in rhythmic process,metabolic process,behavior,regula-tion of biological process,establishment of localization,negative regulation of biological process,biological regulation,lo-calization,cellular process,and multicellular biological process;the cellular component(CC)was mainly concentrated in membrane-enclosed lumen,organelle part,organelle,membrane,extracellular region part,membrane part,cell part,cell;the molecular function(MF)was mainly concentrated in binding,structural molecular activity,and catalytic activi

关 键 词：铁蓄积 差异表达基因 基因本体功能富集 真核生物蛋白相邻类的聚簇分类富集 

分 类 号：R735[医药卫生—肿瘤]