Ⅲ期B/C级牙周炎与IL-18及其多态性位点的相关性分析  

作　　者：张媛媛[1] 丁成[2] 

机构地区：[1]杭州师范大学附属医院,310000 [2]杭州师范大学口腔医学院,310000

出　　处：《浙江临床医学》2024年第4期558-560,共3页Zhejiang Clinical Medical Journal

基　　金：杭州市科技局引导项目(20211231Y028)。

摘　　要：目的探讨牙周炎与IL-18及其多态性位点的相关性。方法检测143例牙周炎患者(观察组)及180例健康体检者(对照组)血浆IL-18水平,分析血浆IL-18与牙周炎的相关性,对IL-18基因rs187238和rs1946518位点进行测序,分析不同基因型与Ⅲ期B/C级牙周炎发病风险的相关性。结果牙周炎患者中血浆IL-18表达水平为(463.06±48.04)pg/mL,高于对照组,差异有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,rs187238位点GC基因型在牙周炎患者中的分布高于对照组(OR=0.36,95%CI:0.21~0.64,P<0.05);rs1946518位点AA基因型在牙周炎患者中的分布高于对照组(OR=0.53,95%CI:0.29~0.96,P<0.05)。rs187238位点分层分析结果显示:在吸烟人群中,该位点杂合基因型GC在牙周炎患者中的频率高于对照组(P<0.05)。单倍型研究显示,牙周炎患者中携带CA和GA单倍型的个体高于对照组(P<0.05),提示携带这两种单倍型可增加牙周炎发病风险。结论IL-18及其遗传变异在牙周炎的发病过程中可能发挥着重要作用。Objective To explore the relationship between periodontitis and IL-18 and its polymorphic sites.Methods The plasma IL-18 level of all subjects was measured,and the relationship between plasma IL-18 and periodontitis was analyzed.To compare the correlation between different genotypes and the risk of periodontitis,we sequenced the rs187238 and rs1946518 loci of IL-18 gene in 323 subjects.Results The level of plasma IL-18 expression in patients with periodontitis was 463.06±48.04pg/ml,which was higher than that in the healthy control group,and the difference was statistically significant(P<0.05).Multivariate logistic regression analysis showed that the distribution of rs187238 GC genotype in patients with periodontitis was significantly higher than that in the healthy controls(OR=0.36,95%CI:0.21~0.64,P<0.05).The distribution of AA genotype at rs1946518 locus in patients with periodontitis was also significantly higher than that in the healthy controls(OR=0.53,95%CI:0.29~0.96,P<0.05).The results of hierarchical analysis of rs187238 locus showed that the frequency of heterozygous genotype GC at this locus in patients with periodontitis was significantly higher than that in the healthy controls(P<0.05).The haplotype study showed that individuals carrying AA haplotypes could increase the risk of periodontitis.The combined effect of genes also found that the risk of periodontitis increased with the increase of carrying risk genes.The haplotype study showed that the individuals carrying CA and GA haplotypes in patients with periodontitis were significantly higher than those in the healthy control group(P<0.05),suggesting that carrying these two haplotypes could increase the risk of periodontitis.Conclusion IL-18 and its genetic variation may play an important role in the pathogenesis of periodontitis in Chinese Han population.

关 键 词：IL-18 牙周炎 基因多态性 相关性 

分 类 号：R781.42[医药卫生—口腔医学]