Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of ovalbumin and alleviates Th2-mediated allergic asthma  

作　　者：Ishfaq Ahmed Suidong Ouyang Shengquan Wu Haochang Song Miaoyuan Zhang Renxing Luo Peishan Lu Jiaqi Deng Tingting Zheng Yanyan Wang Xinguang Liu Gonghua Huang 

机构地区：[1]Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,The First Dongguan Affiliated Hospital,Guangdong Medical University,Dongguan 523808,China [2]Haide College,Ocean University of China,Laoshan Campus,Qingdao 266100,China [3]School of Medical Technology,Guangdong Medical University,Dongguan 523808,China

出　　处：《Food Science and Human Wellness》2024年第4期1962-1973,共12页食品科学与人类健康（英文）

基　　金：supported by the Guangdong Basic and Applied Basic Research Foundation(2021B1515130004;2021B1515140021);the Scientific Research Start-up Funding of Guangdong Medical University(1026/4SG21229G);China Postdoctoral Science Foundation(2021M702781);Guangdong Medical University Post-doctoral Research Funding(2BH19006P)。

摘　　要：Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are inadequate.In this study,we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid(Lac/CA)-catalyzed crosslinking in conjunction with galactomannan(Man).The formation of high molecular weight crosslinked polymers and the Ig G-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting.The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity.Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid(SGF)and simulated intestinal fluid(SIF).Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines(interleukin(IL)-4,IL-5,and IL-13),and upregulated IFN-γand IL-10 expression.Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers(CD80 and CD86)and MHC class II molecules,and suppressed the expression levels of proinflammatory cytokines.The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.

关 键 词：OVALBUMIN LACCASE GALACTOMANNAN Conformational structure Asthma 

分 类 号：R562.25[医药卫生—呼吸系统] TS201.4[医药卫生—内科学]