Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis  

作　　者：Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Research Center for Microbial and Enzyme Engineering Technology,College of Life Science and Technology,Guangxi University,Nanning 530004,China [2]National Engineering Research Center for Non-Food Biorefinery,State Key Laboratory of Non-Food Biomass and Enzyme Technology,Guangxi Research Center for Biological Science and Technology,Guangxi Academy of Sciences,Nanning 530007,China [3]Guangxi Key Laboratory for Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China

出　　处：《Food Science and Human Wellness》2024年第4期2048-2059,共12页食品科学与人类健康（英文）

基　　金：This research was supported by the Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development(GUIKEZY21195021);the Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China(2019GXNSFFA245011);the Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development(20231012);the Funding Projects of Guangxi Key Research and Development Plan(GUIKE AB23075173);the Funding Project of Technological Development from Angel Yeast(Chongzuo)Co.,Ltd.(JS1006020230722019);the Innovation Project of Guangxi Graduate Education(YCBZ2021012).

摘　　要：The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms.Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis.Results showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely found.Hyphal morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were annotated.True hyphae and phospholipase were absent.Only one retroelement(Tad1-65_BG)was found.Major biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined thresholds.GXDK6 had no resistance to common antifungal drugs.Virulence enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were absent.Acute toxicity test with mice demonstrated that GXDK6 is safe.GXDK6 has a good reproduction ability in the simulation gastrointestinal tract.GXDK6 also has a strong antioxidant ability,β-glucosidase,and inulinase activity.To sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

关 键 词：Meyerozyma guilliermondii Safety assessment PROBIOTICS Marine mangrove microorganisms Whole-genome sequencing analysis 

分 类 号：TS201.3[轻工技术与工程—食品科学]