Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus  

作　　者：Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou 

机构地区：[1]Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China [2]Huagongliya(Foshan)Technology Industry Co.,Ltd.,Foshan 528313,China [3]Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517001,China [4]Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China

出　　处：《Food Science and Human Wellness》2024年第4期2161-2173,共13页食品科学与人类健康（英文）

基　　金：the Key Research and Development Program of Yunnan Province(No.202202AE090023);Key Research and Development Program of Guangdong Province(No.2022B0202040002;2022B0202050001);the Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture;the Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture Project(No.DT20220026);Talent Introduction Program of Guangdong Academy of Agricultural Sciences(No.R2022YJ-YB3004);the Basic and Applied Basic Research Project of Guangdong Province(No.2022A1515110227;2023A1515012386);the Science and Technology Planning Project of Guangzhou(No.2023A04J0828);the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams(No.202109TD);the Special Fund Project for Teachers’Scientific and Technological Achievements Transformation in Shunde Innovation Park,National University Science Park,South China University of Technology(No.KJYS2021KZ05)for their financial support。

摘　　要：Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.

关 键 词：Mulberry leaf oligosaccharide Characterization Gut microbiota Lactobacillus murinus Hypoglycemic activity 

分 类 号：TS201.2[轻工技术与工程—食品科学]