利用代谢工程改造解脂耶式酵母合成角黄素  

Modification of Yarrowia lipolytica for the biosynthesis of canthaxanthin by metabolic engineering

作　　者：刘双 窦平川 蒋珊 吴靖叶 万霞 LIU Shuang;DOU Pingchuan;JIANG Shan;WU Jingye;WAN Xia(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]中国农业科学院油料作物研究所,武汉430062 [3]农业部油料作物生物学与遗传改良重点实验室,武汉430062

出　　处：《武汉轻工大学学报》2024年第2期1-10,共10页Journal of Wuhan Polytechnic University

基　　金：国家自然科学基金(编号:32372283).

摘　　要：角黄素是一种橙红色的二酮类胡萝卜素,作为着色剂和抗氧化剂被广泛应用于食品、饲料和化妆品等领域。为生产高水平的角黄素,改造解脂耶氏酵母,通过增加内源牻牛儿基牻牛儿基焦磷酸供给、引入外源β-胡萝卜素生物合成路径、过表达DNA修复基因rad52、增强还原能量及前体物供应,构建了高产β-胡萝卜素的工程菌株。进一步引入副球菌来源的β-胡萝卜素酮化酶基因,增加基因拷贝数及β-胡萝卜素的前体物供应,回补营养缺陷型标签,构建的工程菌株在摇瓶条件下合成了301.1 mg/L的角黄素。实验结果报告了目前微生物合成角黄素的最高摇瓶产量,可为角黄素的商业化生产提供理论指导,同时也为其它类胡萝卜素的生物合成提供理论依据。Canthaxanthin,an orange-red ketocarotenoid,is widely used as a colorant and antioxidant in food,feed,and cosmetic industries.In order to produce high levels of canthaxanthin,Yarrowia lipolytica was modified to construct a highβ-carotene-producing strain achieved by enhancing the flux of endogenous geranylgeranyl diphosphate,strengthening the exogenousβ-carotene biosynthetic pathway,overexpressing the DNA repair gene rad52,and enhancing NADPH supply and precursors.The study further introduced aβ-carotene ketolase gene from Paracoccus sp.,increased gene copy number and the supply ofβ-carotene precursors,and complemented auxotrophic markers.The final engineered strain synthesized 301.1 mg/L of canthaxanthin under shake-flask fermentation conditions.The results of the experiment were reported the highest shake-flask production of canthaxanthin by microorganisms to date,providing theoretical guidance for the commercial production of canthaxanthin and serving as a reference for the synthesis of other carotenoids.

关 键 词：角黄素 解脂耶式酵母 Β-胡萝卜素 

分 类 号：TS213[轻工技术与工程—粮食、油脂及植物蛋白工程]