高产DHA裂殖壶菌的诱变选育及转录组分析  

Mutagenic breeding and transcriptome analysis of Aurantiochytrium limacinum with high yield DHA production

作　　者：蔺楚彬 万霞[2,3,4] 曾驰 LIN Chubin;WAN Xia;ZENG Chi(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062;Key Laboratory of Oilseeds Processing,Ministry of Agriculture,Wuhan 430062)

机构地区：[1]武汉轻工大学生命科学与技术学院,武汉430023 [2]中国农业科学院油料作物研究所,武汉430062 [3]农业部油料作物生物学与遗传改良重点实验室,武汉430062 [4]农业部油料加工重点实验室,武汉430062

出　　处：《武汉轻工大学学报》2024年第2期42-49,55,共9页Journal of Wuhan Polytechnic University

摘　　要：裂殖壶菌(Aurantiochytrium limacinum)可以积累大量的Ω-3多不饱和脂肪酸,特别是高含量的二十二碳六烯酸(DHA),在食品工业中发挥着重要作用。为了获得高产DHA的裂殖壶菌,通过对原始菌株进行电子束(EB)辐射诱变,并结合丙二酸和尼罗红染色法筛选,得到了一个DHA产量提高11.42%的突变体M2-11。转录组结果表明,与原始菌株相比较,M2-11中与脂质合成相关的基因绝大多数被上调,还存在多个差异显著的其他功能基因。本研究为裂殖壶菌的诱变选育和遗传改造提供了参考。Aurantiochytrium limacinum can accumulate large amounts of omega-3 polyunsaturated fatty acids,especially high levels of docosahexaenoic acid,which plays an important role in the food industry.In order to obtain a high DHA-producing strain,a mutant M2-11 with an 11.42%increase in DHA production was obtained in this study by electron-beam(EB)radiation mutagenesis combined with screening by malonate and nile red staining.The results of transcriptomics showed that the vast majority of genes related to lipid synthesis were up-regulated in M2-11 compared to the original strain,and several other functional genes with significant differences also existed.This study provides a reference for mutagenic selection and genetic modification of A.limacinum.

关 键 词：裂殖壶菌 DHA 诱变 转录组 

分 类 号：Q939.97[生物学—微生物学]