基于转录组学和代谢组学分析粗毛纤孔菌L-1中香豆雌酚和补骨脂素的合成途径及潜在调控因子  

Transcriptomics and metabolomics profiling coumestrol and psoralen synthesis pathways and potential regulators in Inonotus hispidus L-1

作　　者：刘艳 黄传书 杨碧文 向舒翔 唐露 黄越 吴均 宋志光 邢康康 LIU Yan;HUANG Chuanshu;YANG Biwen;XIANG Shuxiang;TANG Lu;HUANG Yue;WU Jun;SONG Zhiguang;XING Kangkang(Chongqing Sericulture Science and Technology Research Institute,Chongqing 400700,China;Chongqing Research Institute of Traditional Chinese Medicine,Chongqing 400065,China)

机构地区：[1]重庆市蚕业科学技术研究院,重庆400700 [2]重庆市中药研究院,重庆400065

出　　处：《菌物学报》2024年第4期52-68,共17页Mycosystema

基　　金：supported by the Chongqing Technology Innovation and Application Development Special Program(CSTB2022 TIAD-LDX0005,CSTB2022TIAD-ZXX0049)。

摘　　要：香豆雌酚和补骨脂素作为香豆素的重要衍生物,主要存在于植物中,在抗癌和抗炎方面发挥着重要作用,但在真菌中却少有报道。我们成功分离并培养了一株含有大量多糖和黄酮类化合物的粗毛纤孔菌L-1 Inonotus hispidus L-1。通过代谢组学分析,我们发现在粗毛纤孔菌L-1子实体中香豆雌酚和补骨脂素作为主要成分,其含量明显高于菌丝体和原基。基于转录组学和代谢组学联合分析,阐明了香豆雌酚和补骨脂素在粗毛纤孔菌L-1中的合成途径及其调控基因。研究发现粗毛纤孔菌L-1中香豆雌酚和补骨脂素在合成过程中有一个共同的前体L-苯丙氨酸,而L-苯丙氨酸的合成受糖酵解/葡萄糖生成途径和苯丙氨酸途径的共同调控,其含量在原基和子实体中显著升高。而且以L-苯丙氨酸为前体的P-香豆酸合成途径的两条下游合成途径中的代谢物显著升高,分别为异黄酮类生物合成途径中的2,7,4’-三羟基异黄酮和香豆雌酚,以及香豆素类生物合成途径中的7-羟基香豆素和补骨脂素。而香豆雌酚和补骨脂素的下游代谢物未检测到。通过转录组学进一步证实香豆雌酚和补骨脂素在合成过程中受到APL、APL1和ECM4这3个基因的调控。这项研究结果证实了粗毛纤孔菌L-1作为一种有价值的香豆雌酚和补骨脂素来源在营养保健品开发和应用方面的潜力。Coumestrol and psoralen as important derivatives of coumarins were mainly found in plants and play important roles in anticancer and anti-inflammation but were less reported in fungi.Our study successfully isolated and characterized Inonotus hispidus L-1,a strain with high polysaccharides and flavonoids.Metabolomic analysis indicated that the content of coumestrol and psoralen in basidiomata of I.hispidus L-1 was significantly higher than that in mycelia and primordia.Combining transcriptomics and metabolomics approaches,the synthetic pathways and regulatory genes involved in the production of coumestrol and psoralen were elucidated.The synthesis of coumarin and psoralen in I.hispidus L-1 has a common precursor,L-phenylalanine.L-phenylalanine synthesis was co-regulated by the glycolysis/gluconeogenesis pathway and the phenylalanine pathway,and its levels were significantly elevated in the primordium and basidioma stages.More interestingly,the downstream metabolites in the P-coumarate acid synthesis pathway,by using L-phenylalanine as a precursor,were significantly increased.These downstream metabolites include 2,7,4ʹ-trihydroxyisoflavanone and coumestrol in the isoflavonoid biosynthesis pathway,as well as 7-hydroxycoumarine and psoralen in the coumarin biosynthesis pathway.The synthesis of coumestrol and psoralen was mainly regulated by the phenylalanine ammonia-lyase(APL),APL1,and glutathione S-transferase omega-like 2(ECM4)genes.The results highlight the potential of I.hispidus L-1 as a valuable source of coumarins and psoralens for the development and application of nutraceuticals.

关 键 词：粗毛纤孔菌 转录组学 代谢组学 类黄酮 香豆雌酚 补骨脂素 

分 类 号：S567.3[农业科学—中草药栽培]