水飞蓟素对S.aureus ATCC25923生物膜及其毒力因子的影响  

Effects of silymarin on S.aureus ATCC25923 biofilm and its virulence factors

作　　者：王嘉康 唐浩国[1] 陈静 司启贺 申茹晓 WANG Jiakang;TANG Haoguo;CHEN Jing;SI Qihe;SHEN Ruxiao(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang,Henan 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《食品与机械》2024年第3期26-32,43,共8页Food and Machinery

摘　　要：目的:探究水飞蓟素对S.aureus ATCC25923生物膜及其毒力因子的影响。方法:采用结晶紫染色法检测水飞蓟素对S.aureus ATCC25923生物膜形成的抑制及清除作用,MTT染色法探究水飞蓟素对其生物膜代谢的影响,冻干血浆和无菌脱纤维羊血探究水飞蓟素对其凝固酶表达和溶血活性的影响,同时用光学显微镜观察不同浓度水飞蓟素对其生物膜影响及傅里叶红外分析细菌成分变化。结果:水飞蓟素对S.aureus ATCC25923的最小抑菌质量浓度(MIC)为0.5 mg/mL,MIC浓度下的水飞蓟素对S.aureus ATCC25923生物膜的抑制率达到90%,清除率达到89.2%,且低剂量的水飞蓟素能够显著抑制其溶血活性和凝固酶的表达。结论:水飞蓟素对金黄色葡萄球菌的生物膜形成具有很好的抑制以及清除作用,同时能够显著抑制其毒力因子的表达。Objective:In this study,the effects of silymarin on the biofilm S.aureus ATCC25923 and its virulence factors were investigated.Methods:The inhibition and clearance of silymarin on S.aureus ATCC25923 biofilm formation were detected by crystal violet staining,the effects of silymarin on its biofilm metabolism were investigated by MTT staining,and the effects of silymarin on its coagulase expression and hemolytic activity were investigated by freeze-dried plasma and aseptic defibrinated sheep blood.In parallel,the effects of different concentrations of silymarin on its biofilm and Fourier transform were observed under an optical microscope.Results:The results showed that the minimum inhibitory concentration(MIC)of silymarin against S.aureus ATCC25923 was 0.5 mg/mL,the inhibition rate of silymarin to S.aureus ATCC25923 biofilm at MIC concentration reached 90%,and the clearance rate reached 89.2%,and the low dose of silymarin could significantly inhibit its hemolytic activity and coagulase expression.Conclusion:The outcomes of the experiment demonstrate the great potential applications of silymarin in the areas of natural preservatives and food safety.

关 键 词：水飞蓟素 金黄色葡萄球菌 生物膜 毒力因子 

分 类 号：TS201.3[轻工技术与工程—食品科学]