QuEChERs方法结合SERS技术检测猪肉中氨基糖苷类抗生素残留  

Detection of aminoglycoside antibiotic residues in pork by QuEChERs method combined with SERS technique

作　　者：杨海帆 丁莉 徐妙文 沈康 王煦博 YANG Haifan;DING Li;XU Miaowen;SHEN Kang;WANG Xubo(Yangzhou University School of Medicine,Yangzhou,Jiangsu 225001,China;Affiliated Hospital of Yangzhou University,Yangzhou,Jiangsu 225001,China)

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]扬州大学附属医院,江苏扬州225001

出　　处：《食品与机械》2024年第3期68-74,81,共8页Food and Machinery

基　　金：国家自然科学基金(编号:81701825);江苏省社会发展基金(编号:BE2018684)。

摘　　要：目的:实现猪肉中氨基糖苷类抗生素残留的快速、定量和高通量检测。方法:以PP合成纸为衬底制备基于金纳米花(AuNFs)的方阵排列SERS基底。通过QuEChERs方法对猪肉样品进行前处理,并对其进行SRES检测。结果:采用4-巯基苯甲酸为SERS探针分子,基底表现出良好的均一性、SERS增强效应、重现性和稳定性。475,619 cm-1特征峰处的SERS信号强度分别与硫酸庆大霉素和硫酸新霉素浓度的对数具有良好的线性关系(R 2分别为0.9916,0.9907),最低检测限分别低至1×10^(-9),1×10^(-8) mol/L,并成功应用于猪肉中氨基糖苷类抗生素的快速、定量和高通量检测。结论:试验方法为SERS技术应用于真实肉类样品中抗生素残留检测提供了一种经济、高效、省时和高灵敏的途径。Objective:To achieve rapid,quantitative and high-throughput detection of aminoglycoside antibiotic residues in pork.Methods:Square-array aligned SERS substrates based on Au nanoflowers(AuNFs)were prepared using PP synthetic paper as a substrate.Pork samples were pre-treated by QuEChERs method and subjected to SRES.Results:Using 4-mercaptobenzoic acid as the SERS probe molecule,the substrate exhibited good homogeneity,SERS enhancement effect,reproducibility and stability.the SERS signal intensities at the characteristic peaks at 475,619 cm-1 showed good linear relationships with the logarithms of the concentrations of gentamicin sulphate and neomycin sulphate,respectively(R 2 of 0.9916 and 0.9907,respectively).The limits of detections(LODs)were as low as 1×10^(-9),1×10^(-8) mol/L,respectively,and were successfully applied to the rapid,quantitative and high-throughput determination of aminoglycoside antibiotics in pork.Conclusion:The experimental method provides an economical,efficient,time-saving and highly sensitive way for the application of SERS technology for the detection of antibiotic residues in real meat samples.

关 键 词：表面增强拉曼散射 金纳米花 氨基糖苷类抗生素 硫酸庆大霉素 硫酸新霉素 猪肉 

分 类 号：TS251.51[轻工技术与工程—农产品加工及贮藏工程] O657.37[轻工技术与工程—食品科学与工程]