液相色谱—串联质谱法测定蜂蜜中4种喹诺酮类药物残留量的不确定度评定  

Uncertainty evaluation for the determination of four quinolone residues in honey by liquid chromatography-tandem mass spectrometry

作　　者：杨韵 曹阳 李菁 钟菲菲 左贺 YANG Yun;CAO Yang;LI Jing;ZHONG Feifei;ZUO He(Changsha Institute for Food and Drug Control,Changsha,Hunan 410036,China;National Alcohol Products Quality Inspection and Testing Center〔Hunan〕,Changsha,Hunan 410036,China;Hunan Labor and Human Resources Vocational College,Changsha,Hunan 410100,China)

机构地区：[1]长沙市食品药品检验所,湖南长沙410036 [2]湖南酒类产品质量检验检测中心〔湖南〕,湖南长沙410036 [3]湖南劳动人事职业学院,湖南长沙410100

出　　处：《食品与机械》2024年第3期75-81,共7页Food and Machinery

基　　金：湖南省市场监督管理局科技计划项目(编号:2023KJJH29)。

摘　　要：目的:对液相色谱—串联质谱法测定蜂蜜中4种喹诺酮类药物残留量进行不确定度评定。方法:根据GB 31657.2—2021规定的方法建立数学模型,分析不确定度来源,并评定各标准不确定度的分量。结果:蜂蜜中恩诺沙星、沙拉沙星、氧氟沙星、环丙沙星残留量测量结果不确定度分别为(4.97±0.81),(10.13±1.58),(7.67±1.23),(14.97±2.25)μg/kg(P=95%,k=2)。结论:试验测量不确定度的主要来源为标准溶液配制与稀释过程和标准曲线拟合,在实际工作中应加以控制。Objective:This study aimed to evaluate the determination uncertainty of four quinolones residues in honey by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods:Based on GB 31657.2—2021,a mathematical model for sources of uncertainty evaluation was established.The uncertainty of each variable which affect the result were calculated and the weight of each uncertainty component was assessed.Results:The uncertainties in the determination of enrofloxacin,sarafloxacin,ofloxacin and ciprofloxacin in honey were(4.97±0.81),(10.13±1.58),(7.67±1.23),(14.97±2.25)μg/kg(P=95%,k=2)respectively.Conclusion:The main influencing factors of the determination uncertainty were preparation and dilution process of standard solution and fitting of standard curve,which should be controlled in practical work.

关 键 词：不确定度 液相色谱—串联质谱法 恩诺沙星 沙拉沙星 氧氟沙星 环丙沙星 蜂蜜 

分 类 号：S896.1[农业科学—特种经济动物饲养] O657.63[农业科学—畜牧兽医]