基于转录组测序分析葡萄籽原花青素对HepG2细胞基因及其相关功能的影响  

Effects of grape seed proanthocyanidins on HepG2 cell genes and related functions based on transcriptome sequencing

作　　者：郑万财 党斌[1,2] 张迹 罗巧玉[4] 冯作山 ZHENG Wancai;DANG Bin;ZHANG Ji;LUO Qiaoyu;FENG Zuoshan(Academy of Agriculture and Forestry Sciences of Qinghai University,Qinghai Tibetan Plateau Key Laboratory of Agricultural Products Processing,Xining,Qinghai 810023,China;Qinghai Tibetan Plateau Germplasm Resources Research and Utilization Laboratory,Xining,Qinghai 810023,China;College of Life Sciences,Huaiyin Normal University,Huai'an,Jiangsu 223300,China;College of Life Science,Qinghai Normal University,Xining,Qinhai 810000,China;Food Science and Pharmacy College,Xinjiang Agricultural University,Urumqi,Xinjiang 830000,China)

机构地区：[1]青海大学农林科学院青藏高原种质资源研究与利用实验室,青海西宁810023 [2]青海省青藏高原农产品加工重点实验室,青海西宁810023 [3]淮阴师范学院生命科学学院,江苏淮安223300 [4]青海师范大学生命科学学院,青海西宁810023 [5]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830000

出　　处：《食品与机械》2024年第3期188-195,共8页Food and Machinery

基　　金：青海省科技厅创新平台建设项目(编号:2022-ZJ-T04);青海“昆仑英才·高端创新创业人才”计划特色人才项目(编号:2022-027)。

摘　　要：目的:基于转录组测序分析葡萄籽原花青素对HepG2细胞基因及相关功能的影响,明确原花青素处理HepG2细胞的关键基因及代谢通路。方法:通过转录组测序,筛选差异基因,基因功能注释和KEGG通路的富集分析,进行葡萄籽原花青素处理细胞后的转录组学研究。结果:葡萄籽花青素处理HepG2细胞后主要富集到的生物学过程为细胞过程、代谢过程、生物调控过程、免疫系统过程、繁殖调节、生长调节。细胞凋亡的12个关键差异基因与TNF、p53、MAPK、PI3K-Akt、NF-κB信号通路密切相关。结论:细胞凋亡与TNF信号通路、p53信号传导途径、PI3K/Akt信号通路、NF-κB信号通路、MAPK信号通路密切相关。Objective:Based on transcriptome sequencing analysis,the effect of grape seed procyanidins on HepG2 cell genes and related functions was identified.The key genes and metabolic pathways involved in the treatment of HepG2 cells with procyanidins were confirmed.Methods:Through transcriptome sequencing,screening differential genes,gene functional annotation and enrichment analysis of the KEGG pathway,transcriptome studies were conducted on grape proanthocyanidin-treated cells.Results:After treating HepG2 cells with grape anthocyanins,the main biological processes enriched are cellular processes,metabolic processes,biological regulatory processes,immune system processes,reproductive regulation,and growth regulation.Research has found that 12 key differentially expressed genes in cell apoptosis are associated with TNF,p53,MAPK,PI3K-Akt and NF-κB signaling pathway is closely related.Conclusion:Cell apoptosis is closely related to the TNF signaling pathway,p53 signaling pathway,PI3K/Akt signaling pathway,NF-κB signaling pathway and MAPK signaling pathway.

关 键 词：葡萄籽 原花青素 转录组测序 HEPG2细胞 基因功能 

分 类 号：TS201.4[轻工技术与工程—食品科学]