锑污染物对绿藻及蓝藻的急性毒性效应  

Acute toxic effects of antimony contaminants on green algae and cyanobacteria

作　　者：李方[1,2] 张怡立 王曼 孟祥周[2,3] 沈忱思 LI Fang;ZHANG Yili;WANG Man;MENG Xiangzhou;SHEN Chensi(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China;College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China)

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海污染控制与生态安全研究院,上海200092 [3]同济大学环境科学与工程学院,上海200092

出　　处：《纺织学报》2024年第4期169-179,共11页Journal of Textile Research

基　　金：上海市自然科学基金项目(21ZR1401500);东华大学中央高校基金理工基地项目(2232022G-11/2232023G-11)。

摘　　要：为深入了解不同价态锑的水生生态毒性及其影响因素,选用2种绿藻(羊角月牙藻、莱茵衣藻)和2种蓝藻(聚球藻、水华鱼腥藻)作为模式生物,通过测定藻细胞抑制率、叶绿素a、抗氧化酶及观察细胞微观结构,对锑(Ⅲ)和锑(Ⅴ)进行毒性影响研究。结果表明:在2 mg/L锑(Ⅲ)或锑(V)暴露72 h后,锑(Ⅲ)对微藻的抑制率最高达76.6%,而锑(Ⅴ)对微藻的最高抑制率仅为41.0%,且绿藻较蓝藻受锑胁迫更为敏感;微藻的叶绿素a合成及可溶性蛋白含量变化表现出与生长抑制类似的响应规律,相较之下蓝藻的叶绿素a合成受到的影响较绿藻小;绿藻的超氧化物歧化酶和过氧化氢酶活性变化规律呈现出低浓度促进高浓度抑制的现象,而蓝藻则不具备统一的变化规律;从亚细胞结构来看,莱茵衣藻的细胞壁、细胞核、叶绿体及其它细胞器均受到了损伤,而聚球藻主要是光合系统受损。Objective Antimony(Sb)is utilized as a catalyst during the polymerization process of polyethylene terephthalate(PET)and it remains in PET fibers or textiles.When printing and dyeing PET,Sb will leach into wastewater and cause contamination,and it commonly exists in aquatic environments in two oxidation states:+3 and+5.Investigating their toxicological effects on aquatic ecosystems is highly necessary.Microalgae are the primary producers in aquatic ecosystems,and their short growth cycle and ease of isolation make them highly suitable for studying the toxic effects of Sb pollutants on aquatic ecosystems.Method Representatives of green algae,Raphidocelis subcapitata and Chlamydomonas reinhardtii,and representatives of cyanobacteria,Synechococcus and Dolichospermum sp.,were selected for investigation.The growth status of the microalgae was evaluated by measuring algal cell concentration,chlorophyll a,and soluble protein.The potential oxidative stress caused by exposure to Sb(Ⅲ)and Sb(Ⅴ)was assessed by measuring the activities of superoxide dismutase(SOD)and catalase(CAT).The potential damage to microalgal was determined through subcellular structure observation by TEM.Additionally,the adsorption or absorption of Sb(Ⅲ)and Sb(V)by microalgae were quantified to determine the extent of adsorption relative to their growth status.Results The results revealed that under different concentrations of Sb(Ⅲ)and Sb(Ⅴ)stress for 72 h,Hormesis effect was observed in four algae species,i.e.Raphidocelis subcapitata,Chlamydomonas reinhardtii,Synechococcus,Dolichospermum sp.Sb(Ⅲ)had a more potent inhibitory effect on microalgae,with up to a 76.6% reduction in growth,compared to Sb(Ⅴ)which only resulted in a 41.0%decrease.Green algae were found to be more vulnerable to Sb-induced stress compared to cyanobacteria.The toxic impact of Sb on microalgae was primarily attributed to the impairment of their photosynthetic machinery and the occurrence of oxidative damage.Alterations in the synthesis of chlorophyll a and soluble pr

关 键 词：锑污染 蓝藻 绿藻 毒性效应 废水处理 

分 类 号：X173[环境科学与工程—环境科学]