闽楠挥发性组分提取工艺优化及组学分析  

Optimized extraction and metabolomics analysis of volatile components in Phoebe bournei

作　　者：刘桐语 李荣生[1] 杨锦昌[1] 袁在翔 余纽 吴伟煌 LIU Tongyu;LI Rongsheng;YANG Jinchang;YUAN Zaixiang;YU Niu;WU Weihuang(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520,China;College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037,China)

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]南京林业大学林学院,江苏南京210037

出　　处：《森林与环境学报》2024年第3期289-297,共9页Journal of Forest and Environment

基　　金：中国林业科学研究院中央级公益性科研院所基本科研业务费专项“沉香、猴耳环等树种功能成分分析与质量评价”(CAFYBB2020SY019)。

摘　　要：为优化闽楠挥发性组分水蒸气蒸馏工艺,并探究闽楠各部位挥发性组分差异。以广东省乐昌市闽楠试验林的叶片、枝条、树干及根系4个部位为试验材料,采用水蒸气蒸馏法,开展单因素试验及正交试验,考察离子溶液浓度、料液比及提取时间3个影响因素对挥发性组分产率的影响,确定最佳工艺参数;用气相色谱-质谱联用(GC-MS)技术测定各部位挥发性组分含量。结果表明:(1)叶片挥发性组分最优提取工艺为NaCl浓度45 g·L^(-1)、料液比1∶6、提取时间5 h,产率为0.056%;检测出110种挥发性组分,含量最高的5种组分为β-瑟林烯、β-桉叶醇、双环大根香叶烯、香橙烯、α-衣兰油烯。(2)枝条挥发性组分最优提取工艺为NaCl浓度45 g·L^(-1)、料液比1∶8、提取时间5 h,产率为0.083%;检测出80种挥发性组分,含量最高的5种组分为愈创醇、乙酸愈创木酯、茅苍术醇、γ-桉叶醇和δ-杜松烯。(3)树干挥发性组分最优提取工艺为NaCl浓度30 g·L^(-1)、料液比1∶8、提取时间5 h,产率为0.323%;检测出64种挥发性组分,含量最高的5种组分为茅苍术醇、γ-杜松烯、愈创醇、γ-桉叶醇和马鞭草烯酮。(4)根系挥发性组分最优提取工艺为NaCl浓度30 g·L^(-1)、料液比1∶9、提取时间5 h,产率为1.025%;检测出69种挥发性组分,含量最高的5种组分为茅苍术醇、γ-杜松烯、愈创醇、γ-桉叶醇和马鞭草烯酮。(5)闽楠各部位挥发性组分以萜类和醇类物质居多,占总含量80%以上,4个部位的共有成分仅15种,叶片与其他3个部位的成分差异较大。In order to optimize the steam distillation process of volatile components in Phoebe bournei and explore the differences,four parts(leaves,branches,trunks and roots)from the P.bournei test forest in Lechang City,Guangdong Province were used as materials to conduct single-factor tests and orthogonal tests by steam distillation,aims to investigate the influence of three factors(NaCl concentration,material-liquid ratio to solution and extraction time)on the yield of volatile components,determine the optimum process parameters.The extracts from four parts of P.bournei were determined by GC-MS to explore the content and differences of volatile components.The results showed that:(1)the optimal process combinations for leaves was NaCl concentration 45 g·L^(-1),material-liquid ratio 1∶6,and extraction time 5 h.The extraction rate was 0.056%,110 volatile components detected.The top five fractions were beta-selinene,beta-eudesmol,bicyclogermacrene,aromandendrene and alpha-muurolene.(2)the optimal process combinations for branches was NaCl concentration 45 g·L^(-1),material-liquid ratio 1∶8,and extraction time 5 h.The extraction rate was 0.083%,80 volatile components detected.The top five fractions were guaiol,guaiol acetate,agarospirol,gamma-eudesmol and delta-cadinene.(3)the optimal process combinations for trunks was NaCl concentration 30 g·L^(-1),material-liquid ratio 1∶8,and extraction time 5 h.The extraction rate was 0.323%,64 volatile components detected.The top five fractions were agarospirol,gamma-cadinene,guaiol,gamma-eudesmol and verbenone.(4)the optimal process combinations for roots was NaCl concentration 30 g·L^(-1),material-liquid ratio 1∶9,and extraction time 5 h.The extraction rate was 1.025%,69 volatile components detected.The top five fractions were agarospirol,gamma-cadinene,guaiol,gamma-eudesmol and verbenone.(5)these volatile components were major consisted of terpenes and alcohols,accounting for more than 80%of the total content.Only 15 volatile components were common among leaves,branches

关 键 词：闽楠 挥发性组分 提取工艺 水蒸气蒸馏法 正交试验 气相色谱-质谱联用(GC-MS) 代谢组学 

分 类 号：S792.24[农业科学—林木遗传育种]