磺基水杨酸提取鱼粉中生物胺条件优化研究  

Study on the optimization of extraction conditions for biogenic amine in fish meal with sulfosalicylic scid

作　　者：史莹 吴振洲 刘宇彤 金俐延 赵虹霏 邵彩梅 SHI Ying;WU Zhenzhou;LIU Yutong;JIN Liyan;ZHAO Hongfei;SHAO Caimei(Wellhope Foods Co.,Ltd.,Shenyang,Liaoning Province 110000,China)

机构地区：[1]禾丰食品股份有限公司,辽宁沈阳110000

出　　处：《中国饲料》2024年第9期113-117,共5页China Feed

基　　金：国家生猪产业技术体系建设专用项(CARS-35)。

摘　　要：本实验旨在对磺基水杨酸提取鱼粉中3种生物胺的条件进行优化。通过单因素实验和正交实验考察提取溶液种类、称量质量、提取溶液浓度、超声时间4种因素对生物胺提取效果的影响,并对优化后的条件,进行加标回收率实验及质控样品实验,验证结果的准确性。结果表明:正交实验优选生物胺提取的最佳条件为:称量质量0.5~1.0 g、磺基水杨酸浓度1.0%、超声时间30 min。加标回收率101%~105%,组胺质控样品实测值458 mg/kg,指定值(452.8±64.9)mg/kg,结果可靠。通过磺基水杨酸提取鱼粉中的生物胺、茚三酮作为衍生试剂,氨基酸分析仪检测,达到很好的检测效果,提供了一种简单、快捷的检测方法。The aim of this experiment was to optimize the extraction conditions of three biogenic amines in fish meal with sulfosalicylic acid.Single factor experiment and orthogonal analysis were used to investigate the influence of four factors on the extraction effect of biogenic amine,namely,the type of extraction solution,weighing quality,concentration of extraction solution,and ultrasonic time.For the optimized conditions,the spiked recovery rate experiment and quality control sample experiment were carried out to verify the accuracy of the results.The results showed that the optimum extraction conditions of biogenic amine were selected by orthogonal experiment as follows:weighing 0.5~1.0 g,concentration of sulfosalicylic acid 1.0%,ultrasonic time 30 min.The recovery rate of the spiked sample was 101%~105%.The measured value of the quality control sample was 458 mg/kg,and the specified value was(452.8±64.9)mg/kg.The results were reliable.The biogenic amines in fish meal extracted by sulfosalicylic acid,ninhydrin were used as derivative reagents and detected by amino acid analyzer,which achieved good detection results and provided a simple and fast detection method.

关 键 词：鱼粉 磺基水杨酸 生物胺 正交实验 氨基酸分析仪 

分 类 号：S816.17[农业科学—饲料科学]